Unusual Polyhydroxylated Steroids from the Starfish <i>Anthenoides laevigatus</i>, Collected off the Coastal Waters of Vietnam

• Main Authors: Alla A. Kicha, Dinh T. Ha, Timofey V. Malyarenko, Anatoly I. Kalinovsky, Roman S. Popov, Olesya S. Malyarenko, Tran T. T. Thuy, Pham Q. Long, Nguyen T. T. Ha, Natalia V. Ivanchina
• Format: Article
• Language: English
• Published: MDPI AG 2020-03-01
• Series: Molecules
• Subjects: polyhydroxylated steroids; nmr spectra; starfish; <i>anthenoides laevigatus</i>; cytotoxicity; soft agar assay
• Online Access: https://www.mdpi.com/1420-3049/25/6/1440
• ISSN: 1420-3049

Four new polyhydroxylated steroids <b>1</b>&#8722;<b>4</b> were isolated along with two previously known related steroids <b>5</b> and <b>6</b> from the methanolic extract of the starfish <i>Anthenoides laevigatus</i> collected off the coastal waters of Vietnam. Structures of new compounds were substantially elucidated by one-dimensional (1D) and two-dimensional (2D) NMR spectroscopy and HRESIMS techniques. Heptaol <b>1</b> and hexaol <b>2</b> contain the common 5&#945;-cholestane skeleton, while hexaol <b>3</b> and heptaol <b>4</b> have the rare among starfish steroid compounds 5&#946;-cholestane skeleton. Compounds <b>1</b>, <b>5</b>, and <b>6</b> do not show cytotoxic effects against normal JB6 Cl41 and cancer HT-29 and MDA-MB-231 cells, however they inhibit cell proliferation and colony formation of cancer HT-29 and MDA-MB-231 cells.