Modelation of growth kinetics of mammalian cells in perfusion culture

• Main Authors: Luis Y Hernández, Diaselys Castro, Pablo de la A Vitón, Oscar Pérez, Mercedes Rodríguez
• Format: Article
• Language: English
• Published: Elfos Scientiae
• Series: Biotecnología Aplicada
• Subjects: cultivo en perfusión; filtro rotatorio; células de mamíferos; cinética de crecimiento
• Online Access: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522009000300006&lng=en&tlng=en

Specific equations describing the behavior of cell growth, substrate use and product formation in stirred tank fermentors and cell bank-scale perfusion cultures (30 L working volume) were developed from basic mass balance equations. A third-order polynomial equation was statistically fitted to the restricted cell passage through the screen (0) obtained from experimentally run data to model the behavior of the culture with time, since a clear description of the hydrodynamics of the system has not yet been developed. The results of the simulation of the operation process with the VisSim software application using these equations agreed with the experimental data available. The model was used to analyze the influence of cell concentration, specific growth rate and specific product formation rate on the process during years 2000 and 2001, comparing the results obtained for both periods.