An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity

An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity

In this work, laccase from Trametes versicolor pretreated with copper ion solution was entrapped in copper alginate beads. The presence of laccase in copper alginate beads was verified by Fourier transform infrared (FTIR) spectroscopy. The alginate concentration used was optimized based on the speci...

Full description

Bibliographic Details
Main Authors: Sitong Zhang, Zhuofu Wu, Guang Chen, Zhi Wang
Format: Article
Language:English
Published: MDPI AG 2018-07-01
Series:Catalysts
Subjects:
laccase
copper alginate
entrapment
specific activity
degradation rate
Online Access:http://www.mdpi.com/2073-4344/8/7/286
id doaj-cf37c4f525f34c6d81b2228eb54282c4
record_format Article
spelling doaj-cf37c4f525f34c6d81b2228eb54282c42020-11-25T01:54:12ZengMDPI AGCatalysts2073-43442018-07-018728610.3390/catal8070286catal8070286An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic ActivitySitong Zhang0Zhuofu Wu1Guang Chen2Zhi Wang3College of Life Science, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Science, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Science, Jilin Agricultural University, Changchun 130118, ChinaKey Laboratory of Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130012, ChinaIn this work, laccase from Trametes versicolor pretreated with copper ion solution was entrapped in copper alginate beads. The presence of laccase in copper alginate beads was verified by Fourier transform infrared (FTIR) spectroscopy. The alginate concentration used was optimized based on the specific activity and immobilization yield. After entrapment, laccase presents perfect pH stability and thermal stability with 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as the substrate. Moreover, laccase in copper alginate beads exhibits good reusability during continuous batch operation for removing 2,4-dichlorophenol. More importantly, owing to the coupled effect of copper ion activation and copper alginate entrapment, the entrapped laccase shows a 3.0-fold and a 2.4-fold increase in specific activity and 2,4-DCP degradation rate compared with that of free laccase, respectively.http://www.mdpi.com/2073-4344/8/7/286laccasecopper alginateentrapmentspecific activitydegradation rate
collection DOAJ
language English
format Article
sources DOAJ
author Sitong Zhang
Zhuofu Wu
Guang Chen
Zhi Wang
spellingShingle Sitong Zhang
Zhuofu Wu
Guang Chen
Zhi Wang
An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity
Catalysts
laccase
copper alginate
entrapment
specific activity
degradation rate
author_facet Sitong Zhang
Zhuofu Wu
Guang Chen
Zhi Wang
author_sort Sitong Zhang
title An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity
title_short An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity
title_full An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity
title_fullStr An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity
title_full_unstemmed An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity
title_sort improved method to encapsulate laccase from trametes versicolor with enhanced stability and catalytic activity
publisher MDPI AG
series Catalysts
issn 2073-4344
publishDate 2018-07-01
description In this work, laccase from Trametes versicolor pretreated with copper ion solution was entrapped in copper alginate beads. The presence of laccase in copper alginate beads was verified by Fourier transform infrared (FTIR) spectroscopy. The alginate concentration used was optimized based on the specific activity and immobilization yield. After entrapment, laccase presents perfect pH stability and thermal stability with 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as the substrate. Moreover, laccase in copper alginate beads exhibits good reusability during continuous batch operation for removing 2,4-dichlorophenol. More importantly, owing to the coupled effect of copper ion activation and copper alginate entrapment, the entrapped laccase shows a 3.0-fold and a 2.4-fold increase in specific activity and 2,4-DCP degradation rate compared with that of free laccase, respectively.
topic laccase
copper alginate
entrapment
specific activity
degradation rate
url http://www.mdpi.com/2073-4344/8/7/286
work_keys_str_mv AT sitongzhang animprovedmethodtoencapsulatelaccasefromtrametesversicolorwithenhancedstabilityandcatalyticactivity
AT zhuofuwu animprovedmethodtoencapsulatelaccasefromtrametesversicolorwithenhancedstabilityandcatalyticactivity
AT guangchen animprovedmethodtoencapsulatelaccasefromtrametesversicolorwithenhancedstabilityandcatalyticactivity
AT zhiwang animprovedmethodtoencapsulatelaccasefromtrametesversicolorwithenhancedstabilityandcatalyticactivity
AT sitongzhang improvedmethodtoencapsulatelaccasefromtrametesversicolorwithenhancedstabilityandcatalyticactivity
AT zhuofuwu improvedmethodtoencapsulatelaccasefromtrametesversicolorwithenhancedstabilityandcatalyticactivity
AT guangchen improvedmethodtoencapsulatelaccasefromtrametesversicolorwithenhancedstabilityandcatalyticactivity
AT zhiwang improvedmethodtoencapsulatelaccasefromtrametesversicolorwithenhancedstabilityandcatalyticactivity
_version_ 1724988727923572736

Similar Items