The study of chemical composition of Centaurea diffusa Lam.

• Main Authors: V. I. Mozul, I. I. Aksonova, O. I. Panasenko
• Format: Article
• Language: English
• Published: Zaporozhye State Medical University 2019-12-01
• Series: Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki
• Subjects: centaurea; gas chromatography; plant bioactive compounds
• Online Access: http://pharmed.zsmu.edu.ua/article/view/184190/186252
• ISSN: 2306-8094; 2409-2932

The aim of the work is to study the chemical composition of biologically active compounds of Centaurea diffusa Lam. and the future prospects of its application in the medical and pharmaceutical practice. Materials and methods. The object of the study is the grass of Centaurea diffusa Lam., which was collected in Zaporizhia in July 2018. The method of high-performance gas chromatography on the device Agilent 7890B GC System (Agilent, Santa Clara, CA, USA) with mass spectrometry detector Agilent 5977 BGC / MSD (Agilent, Santa Clara, CA, USA) was used to determine the qualitative and quantitative composition of Centaurea diffusa Lam. Results. It was discovered that raw material of Centaurea diffusa Lam. contains 55 components, which are representatives of different classes of biologically active substances. There are phenols – 9, alcohols – 9, carboxylic acids (saturated and unsaturated) – 8, esters – 7, alkanes – 5, heterocyclic compounds – 4, ketones – 4, terpenes – 3, aldehydes – 2, alkenes – 1, monosaccharides – 2, hydrazides – 1. However, these data differ from the total content of compounds by percentage. Phenols make up 21.45 % and this is the highest indicator, carboxylic acids (saturated and unsaturated) are 19.71 %, and alcohols and esters are 17.63 % and 12.43 % respectively. Retusin constitutes the main part among all found compounds – 15.53 %, 4-hexadecanoic acid (12.25 %) and 2-ethyl-2-(hydroxymethyl)-1,3-propanediol (8.17 %) are on the second and third place. It’s useful to note that hexadecanoic acid 2-hydroxy-1-(hydroxymethyl)ethyl ester is present in quantity 4.3 %. Based on the results obtained, it is possible to consider the further prospects of using Centaurea diffusa Lam. in medical and pharmaceutical practice. Conclusions. The chemical composition of grass of Centaurea diffusa Lam. was first set by the method of gas chromatography with mass spectrometry detector. The presence of 55 biologically active components was determined. The experimental results prove the prospects and relevance of studies the chemical composition of Centaurea diffusa Lam. in order to create new herbal remedies based on it.