An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes

An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes

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Actinomycetes have been the most prolific producer of various kinds of antifungal metabolites, and many of them are described as being produced by polyketide synthetases (pks). We present strain of Actinomycetes producing anticandida isolated from rhizosphere plant for amplification of Pks-I genes....

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Main Authors: A. Alimuddin, Widya Asmara, Jaka Widada, M. Mustofa, Reni Nurjasmi
Format: Article
Language:English
Published: Universitas Gadjah Mada, Yogyakarta 2010-06-01
Series:Indonesian Journal of Biotechnology
Online Access:https://jurnal.ugm.ac.id/ijbiotech/article/view/7817
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spelling doaj-cf1c60853612464e9cf649d7335a42462020-11-25T01:42:31ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412010-06-0115110.22146/ijbiotech.78176533An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genesA. AlimuddinWidya AsmaraJaka WidadaM. MustofaReni NurjasmiActinomycetes have been the most prolific producer of various kinds of antifungal metabolites, and many of them are described as being produced by polyketide synthetases (pks). We present strain of Actinomycetes producing anticandida isolated from rhizosphere plant for amplification of Pks-I genes. The isolate was obtained from Wanagama I Forest UGM Yogyakarta. Gene of seven isolates, from total of 173 isolates, were amplified using degenerate primer to detect the presence of pks genes. One strain that is named Streptomyces sp. GMR-22 was partialy identified as anticandida producing actinomycete. The strain shown the strongest activity against Candida albicans. Based on bioautography assay, one spot active with Rf 0.57 was appeared as bright yellow by cerrium sulphate but it was and not visible on UV254 and 366 lights. Key words : pks genes, anticandida, Streptomyces sp GMR-22, rep-PCR, cajuput rhizospherehttps://jurnal.ugm.ac.id/ijbiotech/article/view/7817
collection DOAJ
language English
format Article
sources DOAJ
author A. Alimuddin
Widya Asmara
Jaka Widada
M. Mustofa
Reni Nurjasmi
spellingShingle A. Alimuddin
Widya Asmara
Jaka Widada
M. Mustofa
Reni Nurjasmi
An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes
Indonesian Journal of Biotechnology
author_facet A. Alimuddin
Widya Asmara
Jaka Widada
M. Mustofa
Reni Nurjasmi
author_sort A. Alimuddin
title An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes
title_short An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes
title_full An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes
title_fullStr An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes
title_full_unstemmed An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes
title_sort actinomycetes producing anticandida isolated from cajuput rhizosphere: partial identification of isolates and amplification of pks-i genes
publisher Universitas Gadjah Mada, Yogyakarta
series Indonesian Journal of Biotechnology
issn 0853-8654
2089-2241
publishDate 2010-06-01
description Actinomycetes have been the most prolific producer of various kinds of antifungal metabolites, and many of them are described as being produced by polyketide synthetases (pks). We present strain of Actinomycetes producing anticandida isolated from rhizosphere plant for amplification of Pks-I genes. The isolate was obtained from Wanagama I Forest UGM Yogyakarta. Gene of seven isolates, from total of 173 isolates, were amplified using degenerate primer to detect the presence of pks genes. One strain that is named Streptomyces sp. GMR-22 was partialy identified as anticandida producing actinomycete. The strain shown the strongest activity against Candida albicans. Based on bioautography assay, one spot active with Rf 0.57 was appeared as bright yellow by cerrium sulphate but it was and not visible on UV254 and 366 lights. Key words : pks genes, anticandida, Streptomyces sp GMR-22, rep-PCR, cajuput rhizosphere
url https://jurnal.ugm.ac.id/ijbiotech/article/view/7817
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