Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.

Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.

We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped...

Full description

Bibliographic Details
Main Authors: Aseefhali Bankapur, R Sagar Krishnamurthy, Elsa Zachariah, Chidangil Santhosh, Basavaraj Chougule, Bhavishna Praveen, Manna Valiathan, Deepak Mathur
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3325966?pdf=render
id doaj-cf002c4fdc7840709133a2381e759253
record_format Article
spelling doaj-cf002c4fdc7840709133a2381e7592532020-11-25T01:53:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3507510.1371/journal.pone.0035075Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.Aseefhali BankapurR Sagar KrishnamurthyElsa ZachariahChidangil SanthoshBasavaraj ChouguleBhavishna PraveenManna ValiathanDeepak MathurWe report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 µg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 µg/ml.http://europepmc.org/articles/PMC3325966?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Aseefhali Bankapur
R Sagar Krishnamurthy
Elsa Zachariah
Chidangil Santhosh
Basavaraj Chougule
Bhavishna Praveen
Manna Valiathan
Deepak Mathur
spellingShingle Aseefhali Bankapur
R Sagar Krishnamurthy
Elsa Zachariah
Chidangil Santhosh
Basavaraj Chougule
Bhavishna Praveen
Manna Valiathan
Deepak Mathur
Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.
PLoS ONE
author_facet Aseefhali Bankapur
R Sagar Krishnamurthy
Elsa Zachariah
Chidangil Santhosh
Basavaraj Chougule
Bhavishna Praveen
Manna Valiathan
Deepak Mathur
author_sort Aseefhali Bankapur
title Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.
title_short Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.
title_full Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.
title_fullStr Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.
title_full_unstemmed Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.
title_sort micro-raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 µg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 µg/ml.
url http://europepmc.org/articles/PMC3325966?pdf=render
work_keys_str_mv AT aseefhalibankapur microramanspectroscopyofsilvernanoparticleinducedstressonopticallytrappedstemcells
AT rsagarkrishnamurthy microramanspectroscopyofsilvernanoparticleinducedstressonopticallytrappedstemcells
AT elsazachariah microramanspectroscopyofsilvernanoparticleinducedstressonopticallytrappedstemcells
AT chidangilsanthosh microramanspectroscopyofsilvernanoparticleinducedstressonopticallytrappedstemcells
AT basavarajchougule microramanspectroscopyofsilvernanoparticleinducedstressonopticallytrappedstemcells
AT bhavishnapraveen microramanspectroscopyofsilvernanoparticleinducedstressonopticallytrappedstemcells
AT mannavaliathan microramanspectroscopyofsilvernanoparticleinducedstressonopticallytrappedstemcells
AT deepakmathur microramanspectroscopyofsilvernanoparticleinducedstressonopticallytrappedstemcells
_version_ 1724990758349438976

Similar Items