Asynchronous replication in oncological patients

• Main Authors: G. F. Mikhailova, V. V. Tsepenko, T. G. Shkavrova, E. V. Goloub
• Format: Article
• Language: Russian
• Published: ABV-press 2018-05-01
• Series: Uspehi Molekulârnoj Onkologii
• Subjects: asynchronous replication; fluorescence in situ hybridization; peripheral blood lymphocyte; cancer biomarker
• Online Access: https://umo.abvpress.ru/jour/article/view/133

Epigenetics is a science studying mechanisms of heritable changes in gene function that occur without a change in the DNA sequence. One of the most important marks of epigenetic misbalance of cell genome is an replication asynchrony of genes with biallelic expression. Interphase fluorescence in situ hybridization (I-FISH) in phytohaemagglutinin-stimulated lymphocytes of peripheral blood is a proper method of estimation of aberrant DNA replication time e. g. DNA replication asynchrony. In this review we analyzed reports referring to asynchronous DNA replication of biallelically expressed genes in lymphocytes of peripheral blood of cancer patients. Analysis shows the DNA replication asynchrony is a nonspecific tumor marker observing both in tumor cells and lymphocytes of peripheral blood in oncohematological patients and patients with solid tumors. It’s stated the frequency of lymphocytes with asynchronous DNA replication of studied genes in cancer patients is increased significantly compares with healthy donors and is enhanced during malignance process. It gives the opportunity of potential using asynchronous replication as molecular genetic marker for cancer patients early revealing.