Long Non-coding RNAs Are Differentially Expressed After Different Exercise Training Programs

BackgroundMolecular regulation related to the health benefits of different exercise modes remains unclear. Long non-coding RNAs (lncRNAs) have emerged as an RNA class with regulatory functions in health and diseases. Here, we analyzed the expression of lncRNAs after different exercise training progr...

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Main Authors: Bernardo Bonilauri, Bruno Dallagiovanna
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-09-01
Series:Frontiers in Physiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fphys.2020.567614/full
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spelling doaj-ceb76cb3cf59464793517c33e2e5c2e32020-11-25T03:47:23ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-09-011110.3389/fphys.2020.567614567614Long Non-coding RNAs Are Differentially Expressed After Different Exercise Training ProgramsBernardo BonilauriBruno DallagiovannaBackgroundMolecular regulation related to the health benefits of different exercise modes remains unclear. Long non-coding RNAs (lncRNAs) have emerged as an RNA class with regulatory functions in health and diseases. Here, we analyzed the expression of lncRNAs after different exercise training programs and their possible modes of action related to physical exercise adaptations.MethodsPublic high-throughput RNA-seq data (skeletal muscle biopsies) were downloaded, and bioinformatics analysis was performed. We primarily analyzed data reports of 12 weeks of resistance training (RT), high-intensity interval training (HIIT), and combined (CT) exercise training. In addition, we analyzed data from 8 weeks of endurance training (ET). Differential expression analysis of lncRNAs was performed, and an adjusted P-value < 0.1 and log2 (fold change) ≥0.5 or ≤−0.5 were set as the cutoff values to identify differentially expressed lncRNAs (DELs).ResultsWe identified 204 DELs after 12 weeks of HIIT, 43 DELs after RT, and 15 DELs after CT. Moreover, 52 lncRNAs were differentially expressed after 8 weeks of ET. The lncRNA expression pattern after physical exercise was very specific, with distinct expression profiles for the different training programs, where few lncRNAs were common among the exercise types. LncRNAs may regulate molecular responses to exercise, such as collagen fibril organization, extracellular matrix organization, myoblast and plasma membrane fusion, skeletal muscle contraction, synaptic transmission, PI3K and TORC regulation, autophagy, and angiogenesis.ConclusionFor the first time, we show that lncRNAs are differentially expressed in skeletal muscle after different physical exercise programs, and these lncRNAs may act in various biological processes related to physical activity adaptations.https://www.frontiersin.org/article/10.3389/fphys.2020.567614/fulllncRNAsexercisetrainingRNA-seqmuscleHIIT
collection DOAJ
language English
format Article
sources DOAJ
author Bernardo Bonilauri
Bruno Dallagiovanna
spellingShingle Bernardo Bonilauri
Bruno Dallagiovanna
Long Non-coding RNAs Are Differentially Expressed After Different Exercise Training Programs
Frontiers in Physiology
lncRNAs
exercise
training
RNA-seq
muscle
HIIT
author_facet Bernardo Bonilauri
Bruno Dallagiovanna
author_sort Bernardo Bonilauri
title Long Non-coding RNAs Are Differentially Expressed After Different Exercise Training Programs
title_short Long Non-coding RNAs Are Differentially Expressed After Different Exercise Training Programs
title_full Long Non-coding RNAs Are Differentially Expressed After Different Exercise Training Programs
title_fullStr Long Non-coding RNAs Are Differentially Expressed After Different Exercise Training Programs
title_full_unstemmed Long Non-coding RNAs Are Differentially Expressed After Different Exercise Training Programs
title_sort long non-coding rnas are differentially expressed after different exercise training programs
publisher Frontiers Media S.A.
series Frontiers in Physiology
issn 1664-042X
publishDate 2020-09-01
description BackgroundMolecular regulation related to the health benefits of different exercise modes remains unclear. Long non-coding RNAs (lncRNAs) have emerged as an RNA class with regulatory functions in health and diseases. Here, we analyzed the expression of lncRNAs after different exercise training programs and their possible modes of action related to physical exercise adaptations.MethodsPublic high-throughput RNA-seq data (skeletal muscle biopsies) were downloaded, and bioinformatics analysis was performed. We primarily analyzed data reports of 12 weeks of resistance training (RT), high-intensity interval training (HIIT), and combined (CT) exercise training. In addition, we analyzed data from 8 weeks of endurance training (ET). Differential expression analysis of lncRNAs was performed, and an adjusted P-value < 0.1 and log2 (fold change) ≥0.5 or ≤−0.5 were set as the cutoff values to identify differentially expressed lncRNAs (DELs).ResultsWe identified 204 DELs after 12 weeks of HIIT, 43 DELs after RT, and 15 DELs after CT. Moreover, 52 lncRNAs were differentially expressed after 8 weeks of ET. The lncRNA expression pattern after physical exercise was very specific, with distinct expression profiles for the different training programs, where few lncRNAs were common among the exercise types. LncRNAs may regulate molecular responses to exercise, such as collagen fibril organization, extracellular matrix organization, myoblast and plasma membrane fusion, skeletal muscle contraction, synaptic transmission, PI3K and TORC regulation, autophagy, and angiogenesis.ConclusionFor the first time, we show that lncRNAs are differentially expressed in skeletal muscle after different physical exercise programs, and these lncRNAs may act in various biological processes related to physical activity adaptations.
topic lncRNAs
exercise
training
RNA-seq
muscle
HIIT
url https://www.frontiersin.org/article/10.3389/fphys.2020.567614/full
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