A fluorimetric micro glycerol method and its application to the determination of serum triglycerides

A fluorimetric micro glycerol method and its application to the determination of serum triglycerides

A fluorimetric method has been developed for the estimation of glycerol in aqueous solution. It utilizes a series of reactions in which glycerol is heated with o-aminophenol in the presence of concentrated sulfuric acid and an oxidizing agent, to form 8-hydroxyquinoline which produces fluorescence o...

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Main Authors: Dennis Mendelsohn, Arnold Antonis
Format: Article
Language:English
Published: Elsevier 1961-01-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520390374
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spelling doaj-cea2c113b6ba468cbe826561e07b8da32021-04-23T06:10:55ZengElsevierJournal of Lipid Research0022-22751961-01-01214550A fluorimetric micro glycerol method and its application to the determination of serum triglyceridesDennis Mendelsohn0Arnold Antonis1Department of Pathology and Microbiology, Division of Chemical Pathology, University of the Witwatersrand, Johannesburg, South Africa; Ernest Oppenheimer Heart Research Unit, South African Institute for Medical Research, Johannesburg, South AfricaDepartment of Pathology and Microbiology, Division of Chemical Pathology, University of the Witwatersrand, Johannesburg, South Africa; Ernest Oppenheimer Heart Research Unit, South African Institute for Medical Research, Johannesburg, South AfricaA fluorimetric method has been developed for the estimation of glycerol in aqueous solution. It utilizes a series of reactions in which glycerol is heated with o-aminophenol in the presence of concentrated sulfuric acid and an oxidizing agent, to form 8-hydroxyquinoline which produces fluorescence on chelation with a divalent metal ion in alkaline solution. Experimental details are given for the estimation of serum triglycerides on phospholipid-free serum lipid extracts. The method can also be used for the estimation of phosphatide glycerol.http://www.sciencedirect.com/science/article/pii/S0022227520390374
collection DOAJ
language English
format Article
sources DOAJ
author Dennis Mendelsohn
Arnold Antonis
spellingShingle Dennis Mendelsohn
Arnold Antonis
A fluorimetric micro glycerol method and its application to the determination of serum triglycerides
Journal of Lipid Research
author_facet Dennis Mendelsohn
Arnold Antonis
author_sort Dennis Mendelsohn
title A fluorimetric micro glycerol method and its application to the determination of serum triglycerides
title_short A fluorimetric micro glycerol method and its application to the determination of serum triglycerides
title_full A fluorimetric micro glycerol method and its application to the determination of serum triglycerides
title_fullStr A fluorimetric micro glycerol method and its application to the determination of serum triglycerides
title_full_unstemmed A fluorimetric micro glycerol method and its application to the determination of serum triglycerides
title_sort fluorimetric micro glycerol method and its application to the determination of serum triglycerides
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1961-01-01
description A fluorimetric method has been developed for the estimation of glycerol in aqueous solution. It utilizes a series of reactions in which glycerol is heated with o-aminophenol in the presence of concentrated sulfuric acid and an oxidizing agent, to form 8-hydroxyquinoline which produces fluorescence on chelation with a divalent metal ion in alkaline solution. Experimental details are given for the estimation of serum triglycerides on phospholipid-free serum lipid extracts. The method can also be used for the estimation of phosphatide glycerol.
url http://www.sciencedirect.com/science/article/pii/S0022227520390374
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AT arnoldantonis afluorimetricmicroglycerolmethodanditsapplicationtothedeterminationofserumtriglycerides
AT dennismendelsohn fluorimetricmicroglycerolmethodanditsapplicationtothedeterminationofserumtriglycerides
AT arnoldantonis fluorimetricmicroglycerolmethodanditsapplicationtothedeterminationofserumtriglycerides
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