A fluorimetric micro glycerol method and its application to the determination of serum triglycerides
A fluorimetric method has been developed for the estimation of glycerol in aqueous solution. It utilizes a series of reactions in which glycerol is heated with o-aminophenol in the presence of concentrated sulfuric acid and an oxidizing agent, to form 8-hydroxyquinoline which produces fluorescence o...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1961-01-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520390374 |
| Summary: | A fluorimetric method has been developed for the estimation of glycerol in aqueous solution. It utilizes a series of reactions in which glycerol is heated with o-aminophenol in the presence of concentrated sulfuric acid and an oxidizing agent, to form 8-hydroxyquinoline which produces fluorescence on chelation with a divalent metal ion in alkaline solution. Experimental details are given for the estimation of serum triglycerides on phospholipid-free serum lipid extracts. The method can also be used for the estimation of phosphatide glycerol. |
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| ISSN: | 0022-2275 |