Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells

The purpose of this work is to study the influence of extracellular ATP (adenosine-3-phosphate), which is an activator of purinergic receptors, on contractive activity of rat aortic ring segments precontracted by α1-adrenoreceptors activation with phenylephrine and evaluate the impact of potassium c...

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Main Authors: S. N. Orlov, L. V. Smagliy, S. V. Gusakova, V. S. Rydchenko, Yu. G. Birulina, A. N. Baikov, V. N. Vasiliyev, G. A. Sukhanova, T. S. Fedorova, T. V. Lasukova
Format: Article
Language:English
Published: Siberian State Medical University (Tomsk) 2017-01-01
Series:Bûlleten' Sibirskoj Mediciny
Subjects:
atp
Online Access:https://bulletin.tomsk.ru/jour/article/view/739
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spelling doaj-ce9df3fdb393468297d14999a6f15cd32021-07-29T08:38:03ZengSiberian State Medical University (Tomsk)Bûlleten' Sibirskoj Mediciny1682-03631819-36842017-01-0115510511210.20538/1682-0363-2016-5-105-112606Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cellsS. N. Orlov0L. V. Smagliy1S. V. Gusakova2V. S. Rydchenko3Yu. G. Birulina4A. N. Baikov5V. N. Vasiliyev6G. A. Sukhanova7T. S. Fedorova8T. V. Lasukova9Siberian State Medical University; Lomonosov Moscow State UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversityTomsk State Pedagogical UniversityThe purpose of this work is to study the influence of extracellular ATP (adenosine-3-phosphate), which is an activator of purinergic receptors, on contractive activity of rat aortic ring segments precontracted by α1-adrenoreceptors activation with phenylephrine and evaluate the impact of potassium channels of plasma membrane on mechanisms of ATP activity.Material and methods. Contractive activity of vascular smooth muscle cells was studied using the method of Organ Bath Myography applied to the thoracic aorta segments in male Wistar rats both with intact and removed endothelium. ATP (1–1000 mM) produced a dose-dependent relaxing effect on intact and endothelium-denuded segments precontracted with phenilephrine. To assess the impact of potassium channels on mechanisms of ATP activity we used tetraethylammonium (10 mM), a nonselective potassium-channel blocker, glibenclamide (10 mM), the blocker of ATP-sensitive potassium channels and 4-aminopiridine, a blocker of voltage-gated potassium channels.Conclusion. Our study has shown that the impact of ATP on segments with intact endothelium depends on the ATP-sensitive potassium channels whereas the impact of ATP on endothelium-denuded aortic segments depends on both ATP-sensitive and voltage-gated potassium channels.https://bulletin.tomsk.ru/jour/article/view/739atppotassium channelssmooth muscle cells
collection DOAJ
language English
format Article
sources DOAJ
author S. N. Orlov
L. V. Smagliy
S. V. Gusakova
V. S. Rydchenko
Yu. G. Birulina
A. N. Baikov
V. N. Vasiliyev
G. A. Sukhanova
T. S. Fedorova
T. V. Lasukova
spellingShingle S. N. Orlov
L. V. Smagliy
S. V. Gusakova
V. S. Rydchenko
Yu. G. Birulina
A. N. Baikov
V. N. Vasiliyev
G. A. Sukhanova
T. S. Fedorova
T. V. Lasukova
Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells
Bûlleten' Sibirskoj Mediciny
atp
potassium channels
smooth muscle cells
author_facet S. N. Orlov
L. V. Smagliy
S. V. Gusakova
V. S. Rydchenko
Yu. G. Birulina
A. N. Baikov
V. N. Vasiliyev
G. A. Sukhanova
T. S. Fedorova
T. V. Lasukova
author_sort S. N. Orlov
title Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells
title_short Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells
title_full Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells
title_fullStr Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells
title_full_unstemmed Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells
title_sort role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells
publisher Siberian State Medical University (Tomsk)
series Bûlleten' Sibirskoj Mediciny
issn 1682-0363
1819-3684
publishDate 2017-01-01
description The purpose of this work is to study the influence of extracellular ATP (adenosine-3-phosphate), which is an activator of purinergic receptors, on contractive activity of rat aortic ring segments precontracted by α1-adrenoreceptors activation with phenylephrine and evaluate the impact of potassium channels of plasma membrane on mechanisms of ATP activity.Material and methods. Contractive activity of vascular smooth muscle cells was studied using the method of Organ Bath Myography applied to the thoracic aorta segments in male Wistar rats both with intact and removed endothelium. ATP (1–1000 mM) produced a dose-dependent relaxing effect on intact and endothelium-denuded segments precontracted with phenilephrine. To assess the impact of potassium channels on mechanisms of ATP activity we used tetraethylammonium (10 mM), a nonselective potassium-channel blocker, glibenclamide (10 mM), the blocker of ATP-sensitive potassium channels and 4-aminopiridine, a blocker of voltage-gated potassium channels.Conclusion. Our study has shown that the impact of ATP on segments with intact endothelium depends on the ATP-sensitive potassium channels whereas the impact of ATP on endothelium-denuded aortic segments depends on both ATP-sensitive and voltage-gated potassium channels.
topic atp
potassium channels
smooth muscle cells
url https://bulletin.tomsk.ru/jour/article/view/739
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AT lvsmagliy roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells
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AT vsrydchenko roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells
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AT anbaikov roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells
AT vnvasiliyev roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells
AT gasukhanova roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells
AT tsfedorova roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells
AT tvlasukova roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells
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