Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells
The purpose of this work is to study the influence of extracellular ATP (adenosine-3-phosphate), which is an activator of purinergic receptors, on contractive activity of rat aortic ring segments precontracted by α1-adrenoreceptors activation with phenylephrine and evaluate the impact of potassium c...
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Siberian State Medical University (Tomsk)
2017-01-01
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Online Access: | https://bulletin.tomsk.ru/jour/article/view/739 |
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doaj-ce9df3fdb393468297d14999a6f15cd32021-07-29T08:38:03ZengSiberian State Medical University (Tomsk)Bûlleten' Sibirskoj Mediciny1682-03631819-36842017-01-0115510511210.20538/1682-0363-2016-5-105-112606Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cellsS. N. Orlov0L. V. Smagliy1S. V. Gusakova2V. S. Rydchenko3Yu. G. Birulina4A. N. Baikov5V. N. Vasiliyev6G. A. Sukhanova7T. S. Fedorova8T. V. Lasukova9Siberian State Medical University; Lomonosov Moscow State UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversitySiberian State Medical UniversityTomsk State Pedagogical UniversityThe purpose of this work is to study the influence of extracellular ATP (adenosine-3-phosphate), which is an activator of purinergic receptors, on contractive activity of rat aortic ring segments precontracted by α1-adrenoreceptors activation with phenylephrine and evaluate the impact of potassium channels of plasma membrane on mechanisms of ATP activity.Material and methods. Contractive activity of vascular smooth muscle cells was studied using the method of Organ Bath Myography applied to the thoracic aorta segments in male Wistar rats both with intact and removed endothelium. ATP (1–1000 mM) produced a dose-dependent relaxing effect on intact and endothelium-denuded segments precontracted with phenilephrine. To assess the impact of potassium channels on mechanisms of ATP activity we used tetraethylammonium (10 mM), a nonselective potassium-channel blocker, glibenclamide (10 mM), the blocker of ATP-sensitive potassium channels and 4-aminopiridine, a blocker of voltage-gated potassium channels.Conclusion. Our study has shown that the impact of ATP on segments with intact endothelium depends on the ATP-sensitive potassium channels whereas the impact of ATP on endothelium-denuded aortic segments depends on both ATP-sensitive and voltage-gated potassium channels.https://bulletin.tomsk.ru/jour/article/view/739atppotassium channelssmooth muscle cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
S. N. Orlov L. V. Smagliy S. V. Gusakova V. S. Rydchenko Yu. G. Birulina A. N. Baikov V. N. Vasiliyev G. A. Sukhanova T. S. Fedorova T. V. Lasukova |
spellingShingle |
S. N. Orlov L. V. Smagliy S. V. Gusakova V. S. Rydchenko Yu. G. Birulina A. N. Baikov V. N. Vasiliyev G. A. Sukhanova T. S. Fedorova T. V. Lasukova Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells Bûlleten' Sibirskoj Mediciny atp potassium channels smooth muscle cells |
author_facet |
S. N. Orlov L. V. Smagliy S. V. Gusakova V. S. Rydchenko Yu. G. Birulina A. N. Baikov V. N. Vasiliyev G. A. Sukhanova T. S. Fedorova T. V. Lasukova |
author_sort |
S. N. Orlov |
title |
Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
title_short |
Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
title_full |
Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
title_fullStr |
Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
title_full_unstemmed |
Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
title_sort |
role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells |
publisher |
Siberian State Medical University (Tomsk) |
series |
Bûlleten' Sibirskoj Mediciny |
issn |
1682-0363 1819-3684 |
publishDate |
2017-01-01 |
description |
The purpose of this work is to study the influence of extracellular ATP (adenosine-3-phosphate), which is an activator of purinergic receptors, on contractive activity of rat aortic ring segments precontracted by α1-adrenoreceptors activation with phenylephrine and evaluate the impact of potassium channels of plasma membrane on mechanisms of ATP activity.Material and methods. Contractive activity of vascular smooth muscle cells was studied using the method of Organ Bath Myography applied to the thoracic aorta segments in male Wistar rats both with intact and removed endothelium. ATP (1–1000 mM) produced a dose-dependent relaxing effect on intact and endothelium-denuded segments precontracted with phenilephrine. To assess the impact of potassium channels on mechanisms of ATP activity we used tetraethylammonium (10 mM), a nonselective potassium-channel blocker, glibenclamide (10 mM), the blocker of ATP-sensitive potassium channels and 4-aminopiridine, a blocker of voltage-gated potassium channels.Conclusion. Our study has shown that the impact of ATP on segments with intact endothelium depends on the ATP-sensitive potassium channels whereas the impact of ATP on endothelium-denuded aortic segments depends on both ATP-sensitive and voltage-gated potassium channels. |
topic |
atp potassium channels smooth muscle cells |
url |
https://bulletin.tomsk.ru/jour/article/view/739 |
work_keys_str_mv |
AT snorlov roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells AT lvsmagliy roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells AT svgusakova roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells AT vsrydchenko roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells AT yugbirulina roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells AT anbaikov roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells AT vnvasiliyev roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells AT gasukhanova roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells AT tsfedorova roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells AT tvlasukova roleofpotassiumconductanceinmechanismsofexrtacellularatpimpactonthecontractiveactivityofvascularsmoothmusclecells |
_version_ |
1721253409663746048 |