The effect of 6% Hydroxyethyl starch vs. Ringer's lactate on acute kidney injury after renal ischemia in rats

• Main Authors: Vera Lucia Fernandes de Azevedo, Paulo Sergio Santana Santos, Gildàsio Silveira de Oliveira Jr, Gabriel Pinheiro Módolo, Maria Aparecida Custódio Domingues, Yara Marcondes Machado Castiglia, Pedro Thadeu Galvão Vianna, Luiz Antonio Vane, Norma Sueli Pinheiro Módolo
• Format: Article
• Language: English
• Published: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2013-01-01
• Series: Acta Cirurgica Brasileira
• Subjects: Acute Kidney Injury; Fluid Therapy; Colloids; Hetastarch; Rats
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000100002
• ISSN: 0102-8650; 1678-2674

PURPOSE: To compare fluid replacement therapy with Hydroxyethyl starch 6% (HES) versus Ringer's lactate (RL) in a rodent model of non-septic renal ischemia. METHODS: Forty male Wistar rats were randomized to receive HES 2 ml.kg-1.hr-1or RL 5 ml. kg-1.hr-1 that underwent 30 minutes of renal ischemia followed by reperfusion. Twelve hours after kidney ischemia, the kidneys were evaluated for histological changes. Serum NGAL levels were obtained at different times of the experimental protocol. RESULTS: Rodents in the HES group had a median (IQR) grade of renal injury 3 (3 to 5) compared to 2 (2 to 4) in the RL group (p=0.03). NGAL levels were not associated with the severity of kidney injury. CONCLUSION: Hydroxyethyl starch administration caused more kidney injury than Ringer's lactate in a non-infectious model of renal hypoperfusion.