Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins
Abstract Background The economic viability of a protein-production process relies highly on the production titer and the price of raw materials. Crude glycerol coming from the production of biodiesel is a renewable and cost-effective resource. However, glycerol is inefficiently utilized by Escherich...
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Online Access: | https://doi.org/10.1186/s13068-020-01848-z |
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doaj-ce5c783556614bbf97f2b7b4482f433c2020-12-20T12:39:29ZengBMCBiotechnology for Biofuels1754-68342020-12-011311910.1186/s13068-020-01848-zRewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteinsChung-Jen Chiang0Yi-Jing Ho1Mu-Chen Hu2Yun-Peng Chao3Department of Medical Laboratory Science and Biotechnology, China Medical UniversityDepartment of Chemical Engineering, Feng Chia UniversityDepartment of Chemical Engineering, Feng Chia UniversityDepartment of Chemical Engineering, Feng Chia UniversityAbstract Background The economic viability of a protein-production process relies highly on the production titer and the price of raw materials. Crude glycerol coming from the production of biodiesel is a renewable and cost-effective resource. However, glycerol is inefficiently utilized by Escherichia coli. Results This issue was addressed by rewiring glycerol metabolism for redistribution of the metabolic flux. Key steps in central metabolism involving the glycerol dissimilation pathway, the pentose phosphate pathway, and the tricarboxylic acid cycle were pinpointed and manipulated to provide precursor metabolites and energy. As a result, the engineered E. coli strain displayed a 9- and 30-fold increase in utilization of crude glycerol and production of the target protein, respectively. Conclusions The result indicates that the present method of metabolic engineering is useful and straightforward for efficient adjustment of the flux distribution in glycerol metabolism. The practical application of this methodology in biorefinery and the related field would be acknowledged.https://doi.org/10.1186/s13068-020-01848-zMetabolic engineeringCrude glycerolRecombinant protein |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chung-Jen Chiang Yi-Jing Ho Mu-Chen Hu Yun-Peng Chao |
spellingShingle |
Chung-Jen Chiang Yi-Jing Ho Mu-Chen Hu Yun-Peng Chao Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins Biotechnology for Biofuels Metabolic engineering Crude glycerol Recombinant protein |
author_facet |
Chung-Jen Chiang Yi-Jing Ho Mu-Chen Hu Yun-Peng Chao |
author_sort |
Chung-Jen Chiang |
title |
Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins |
title_short |
Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins |
title_full |
Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins |
title_fullStr |
Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins |
title_full_unstemmed |
Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins |
title_sort |
rewiring of glycerol metabolism in escherichia coli for effective production of recombinant proteins |
publisher |
BMC |
series |
Biotechnology for Biofuels |
issn |
1754-6834 |
publishDate |
2020-12-01 |
description |
Abstract Background The economic viability of a protein-production process relies highly on the production titer and the price of raw materials. Crude glycerol coming from the production of biodiesel is a renewable and cost-effective resource. However, glycerol is inefficiently utilized by Escherichia coli. Results This issue was addressed by rewiring glycerol metabolism for redistribution of the metabolic flux. Key steps in central metabolism involving the glycerol dissimilation pathway, the pentose phosphate pathway, and the tricarboxylic acid cycle were pinpointed and manipulated to provide precursor metabolites and energy. As a result, the engineered E. coli strain displayed a 9- and 30-fold increase in utilization of crude glycerol and production of the target protein, respectively. Conclusions The result indicates that the present method of metabolic engineering is useful and straightforward for efficient adjustment of the flux distribution in glycerol metabolism. The practical application of this methodology in biorefinery and the related field would be acknowledged. |
topic |
Metabolic engineering Crude glycerol Recombinant protein |
url |
https://doi.org/10.1186/s13068-020-01848-z |
work_keys_str_mv |
AT chungjenchiang rewiringofglycerolmetabolisminescherichiacoliforeffectiveproductionofrecombinantproteins AT yijingho rewiringofglycerolmetabolisminescherichiacoliforeffectiveproductionofrecombinantproteins AT muchenhu rewiringofglycerolmetabolisminescherichiacoliforeffectiveproductionofrecombinantproteins AT yunpengchao rewiringofglycerolmetabolisminescherichiacoliforeffectiveproductionofrecombinantproteins |
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1724376305419419648 |