Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins

Abstract Background The economic viability of a protein-production process relies highly on the production titer and the price of raw materials. Crude glycerol coming from the production of biodiesel is a renewable and cost-effective resource. However, glycerol is inefficiently utilized by Escherich...

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Main Authors: Chung-Jen Chiang, Yi-Jing Ho, Mu-Chen Hu, Yun-Peng Chao
Format: Article
Language:English
Published: BMC 2020-12-01
Series:Biotechnology for Biofuels
Subjects:
Online Access:https://doi.org/10.1186/s13068-020-01848-z
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spelling doaj-ce5c783556614bbf97f2b7b4482f433c2020-12-20T12:39:29ZengBMCBiotechnology for Biofuels1754-68342020-12-011311910.1186/s13068-020-01848-zRewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteinsChung-Jen Chiang0Yi-Jing Ho1Mu-Chen Hu2Yun-Peng Chao3Department of Medical Laboratory Science and Biotechnology, China Medical UniversityDepartment of Chemical Engineering, Feng Chia UniversityDepartment of Chemical Engineering, Feng Chia UniversityDepartment of Chemical Engineering, Feng Chia UniversityAbstract Background The economic viability of a protein-production process relies highly on the production titer and the price of raw materials. Crude glycerol coming from the production of biodiesel is a renewable and cost-effective resource. However, glycerol is inefficiently utilized by Escherichia coli. Results This issue was addressed by rewiring glycerol metabolism for redistribution of the metabolic flux. Key steps in central metabolism involving the glycerol dissimilation pathway, the pentose phosphate pathway, and the tricarboxylic acid cycle were pinpointed and manipulated to provide precursor metabolites and energy. As a result, the engineered E. coli strain displayed a 9- and 30-fold increase in utilization of crude glycerol and production of the target protein, respectively. Conclusions The result indicates that the present method of metabolic engineering is useful and straightforward for efficient adjustment of the flux distribution in glycerol metabolism. The practical application of this methodology in biorefinery and the related field would be acknowledged.https://doi.org/10.1186/s13068-020-01848-zMetabolic engineeringCrude glycerolRecombinant protein
collection DOAJ
language English
format Article
sources DOAJ
author Chung-Jen Chiang
Yi-Jing Ho
Mu-Chen Hu
Yun-Peng Chao
spellingShingle Chung-Jen Chiang
Yi-Jing Ho
Mu-Chen Hu
Yun-Peng Chao
Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins
Biotechnology for Biofuels
Metabolic engineering
Crude glycerol
Recombinant protein
author_facet Chung-Jen Chiang
Yi-Jing Ho
Mu-Chen Hu
Yun-Peng Chao
author_sort Chung-Jen Chiang
title Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins
title_short Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins
title_full Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins
title_fullStr Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins
title_full_unstemmed Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins
title_sort rewiring of glycerol metabolism in escherichia coli for effective production of recombinant proteins
publisher BMC
series Biotechnology for Biofuels
issn 1754-6834
publishDate 2020-12-01
description Abstract Background The economic viability of a protein-production process relies highly on the production titer and the price of raw materials. Crude glycerol coming from the production of biodiesel is a renewable and cost-effective resource. However, glycerol is inefficiently utilized by Escherichia coli. Results This issue was addressed by rewiring glycerol metabolism for redistribution of the metabolic flux. Key steps in central metabolism involving the glycerol dissimilation pathway, the pentose phosphate pathway, and the tricarboxylic acid cycle were pinpointed and manipulated to provide precursor metabolites and energy. As a result, the engineered E. coli strain displayed a 9- and 30-fold increase in utilization of crude glycerol and production of the target protein, respectively. Conclusions The result indicates that the present method of metabolic engineering is useful and straightforward for efficient adjustment of the flux distribution in glycerol metabolism. The practical application of this methodology in biorefinery and the related field would be acknowledged.
topic Metabolic engineering
Crude glycerol
Recombinant protein
url https://doi.org/10.1186/s13068-020-01848-z
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AT muchenhu rewiringofglycerolmetabolisminescherichiacoliforeffectiveproductionofrecombinantproteins
AT yunpengchao rewiringofglycerolmetabolisminescherichiacoliforeffectiveproductionofrecombinantproteins
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