| Summary: | Background. Neonols are known as ethoxylated phenol derivatives which have surfactant properties and adversely affect the structure and function of organs by changes in biochemical reactions in the body. Objective. The purpose of this study was the morphological characteristics of liver, kidneys, spleen, adrenal, lung, small intestine, stomach, pancreas, heart of rats under load with various doses of toxic phenol derivatives for a prolonged experiment. Methods. Tissues were fixed in neutral formalin, dehydrated in alcohol and embedded in paraffin. Staining of sections with hematoxylin and eosin was performed according to standard procedures. The 10 mkm sections of organs frozen in liquid nitrogen at a temperature of -196°C were prepared in a cryostat at -18 ° C. In these sections cytophotometric study determined dehydrogenase by tetrazolium salts. Results. It was found that at a dose of 1/1000 DL50 morphological changes in organs are not observed. The oral administration of aqueous solutions of phenol derivatives in doses of 1/10 and 1/100 DL50 caused pathological changes in liver morphology, kidney, spleen and adrenal glands. As a result of the long-term experiment the expanded pericapillary spaces of the liver, deformation of the kidney glomeruli, dilatation of lymphoid follicles of the spleen and enlargement of sinusoidal capillaries in adrenal glands were revealed. Histochemically phenol derivatives increased histological enzymatic activity in the liver, kidneys, adrenal glands, spleen or lead to a modification of activity in the structural units of the organ. Conclusion. The prolonged intoxication by phenol derivatives is critical factor for the development of disorders in structural integrity of tissues in liver, kidneys, spleen, adrenal glands and their further dysfunction.
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