A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells

A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells

Melanoma is an aggressive skin tumor that shows a high mortality rate and level of metastasis. BRAF gene mutation (BRAF V600E) is directly related to the occurrence of melanoma. In this study, a light-inducible gene expression system was designed to control the Cas9 transcription, which could then c...

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Main Authors: Xia Wu, Haiyan Huang, Bo Yu, Jianzhong Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-11-01
Series:Frontiers in Molecular Biosciences
Subjects:
melanoma
light-inducible system
CRISPR/Cas9
gene switch
BRAF V600E
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2020.606593/full
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spelling doaj-ce4f36a9e1c843f7bd19e400b0e531652020-11-25T04:11:22ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2020-11-01710.3389/fmolb.2020.606593606593A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma CellsXia Wu0Xia Wu1Haiyan Huang2Bo Yu3Jianzhong Zhang4Department of Dermatology, Peking University People’s Hospital, Beijing, ChinaDepartment of Dermatology, Skin Research Institute of Peking University Shenzhen Hospital, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Dermatology, Skin Research Institute of Peking University Shenzhen Hospital, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Dermatology, Skin Research Institute of Peking University Shenzhen Hospital, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Dermatology, Peking University People’s Hospital, Beijing, ChinaMelanoma is an aggressive skin tumor that shows a high mortality rate and level of metastasis. BRAF gene mutation (BRAF V600E) is directly related to the occurrence of melanoma. In this study, a light-inducible gene expression system was designed to control the Cas9 transcription, which could then cleave the BRAF V600E. To prove the potential utility of this system in melanoma, the physiological function of melanoma cells was tested. It illustrated that the light-induced CRISPR-Cas9 system could inhibit the progression of G361 and A375 cells. Thus, this system may provide a novel therapeutic strategy of melanoma intervention.https://www.frontiersin.org/articles/10.3389/fmolb.2020.606593/fullmelanomalight-inducible systemCRISPR/Cas9gene switchBRAF V600E
collection DOAJ
language English
format Article
sources DOAJ
author Xia Wu
Xia Wu
Haiyan Huang
Bo Yu
Jianzhong Zhang
spellingShingle Xia Wu
Xia Wu
Haiyan Huang
Bo Yu
Jianzhong Zhang
A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
Frontiers in Molecular Biosciences
melanoma
light-inducible system
CRISPR/Cas9
gene switch
BRAF V600E
author_facet Xia Wu
Xia Wu
Haiyan Huang
Bo Yu
Jianzhong Zhang
author_sort Xia Wu
title A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
title_short A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
title_full A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
title_fullStr A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
title_full_unstemmed A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells
title_sort blue light-inducible crispr-cas9 system for inhibiting progression of melanoma cells
publisher Frontiers Media S.A.
series Frontiers in Molecular Biosciences
issn 2296-889X
publishDate 2020-11-01
description Melanoma is an aggressive skin tumor that shows a high mortality rate and level of metastasis. BRAF gene mutation (BRAF V600E) is directly related to the occurrence of melanoma. In this study, a light-inducible gene expression system was designed to control the Cas9 transcription, which could then cleave the BRAF V600E. To prove the potential utility of this system in melanoma, the physiological function of melanoma cells was tested. It illustrated that the light-induced CRISPR-Cas9 system could inhibit the progression of G361 and A375 cells. Thus, this system may provide a novel therapeutic strategy of melanoma intervention.
topic melanoma
light-inducible system
CRISPR/Cas9
gene switch
BRAF V600E
url https://www.frontiersin.org/articles/10.3389/fmolb.2020.606593/full
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