Functional Multiple-Spine Calcium Imaging from Brain Slices

Summary: Most excitatory inputs arrive at dendritic spines in a postsynaptic neuron. To understand dendritic information processing, it is critical to scrutinize the spatiotemporal dynamics of synaptic inputs along dendrites. This protocol combines spinning-disk confocal imaging with whole-cell patc...

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Bibliographic Details
Main Authors: Tomoe Ishikawa, Chiaki Kobayashi, Naoya Takahashi, Yuji Ikegaya
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301088