LAMP real-time turbidity detection for fowl adenovirus

• Main Authors: Xiao-Yuan Yuan, You-Ling Wang, Kai Meng, Yu-Xia Zhang, Huai-Ying Xu, Wu Ai
• Format: Article
• Language: English
• Published: BMC 2019-07-01
• Series: BMC Veterinary Research
• Subjects: Adenovirus; LAMP; Real-time turbidity; Visual detection
• Online Access: http://link.springer.com/article/10.1186/s12917-019-2015-5

Abstract Background Fowl adenovirus (FAdV) is an infectious agent that can cause jaundice, severe anaemia, dyspnoea and reproductive disorders in fowls. Since 2015, FAdV disease has been rapidly spreading among broiler chickens in China, where it has caused significant economic losses. In this study, a loop-mediated isothermal amplification (LAMP) real-time turbidity method with strong specificity to FAdV was established. Results The established assay was specific to FAdV-4, and the lower limit of detection was 75 copies/μL of extracted DNA. The positive detection rate for the suspected tissue samples was 33.3% (14/42), which was consistent with that of the real-time PCR. Conclusion The proposed LAMP method can quickly and accurately detect prevalent FAdV via real-time turbidity assay, thereby providing a diagnostic platform for the prevention and control of the FAdV disease.