Minerals consumption by Acetobacter xylinum on cultivation medium on coconut water

• Main Authors: Denise Milleo Almeida, Rosilene Aparecida Prestes, Adriel Ferreira da Fonseca, Adenise L. Woiciechowski, Gilvan Wosiacki
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Microbiologia 2013-01-01
• Series: Brazilian Journal of Microbiology
• Subjects: minerals; bacterial cellulose; Acetobacter xylinum
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100030

The objective of this work is to verifying the consume of the minerals K, Na, Fe, Mg, P, S-SO4-2,B,N Total Kjedahl (NTK), NO3--N, and NH4+-N in the production of bacterial cellulose by Acetobacter xylinum, according to the medium and the manner of cultivation. The fermentative process was in ripe and green coconut water. K and Na were determined by flame emission photometry, Mg and Fe by atomic absorption spectrophotometry, P by molecular absorption spectrophotometry, S-SO4-2 by barium sulphate turbidimetry, B by Azomethin-H method, NTK by Kjeldahl method, N-NO3-and N-NH4+ by vapor distillation with magnesium oxide and Devarda's alloy, respectively. In Fermentation of ripe coconut water there were higher consumption of K (69%), Fe (84,3%), P (97,4%), S-SO2-2 (64,9%), B (56,1%), N-NO3 (94,7%) and N-NH4+ (95,2%), whereas coconut water of green fruit the most consumed ions were Na (94,5%), Mg (67,7%) and NTK (56,6%). The cultivation under agitation showed higher mineral consumption. The higher bacterial cellulose production, 6 g.L-1, was verified in the coconut water fermentative in ripe fruit, added KH2PO4, FeSO4 and NaH2PO4 kept under agitation.