ROCK inhibitor is not required for embryoid body formation from singularized human embryonic stem cells.
We report a technology to form human embryoid bodies (hEBs) from singularized human embryonic stem cells (hESCs) without the use of the p160 rho-associated coiled-coil kinase inhibitor (ROCKi) or centrifugation (spin). hEB formation was tested under four conditions: +ROCKi/+spin, +ROCKi/-spin, -ROCK...
Main Authors: | Giuseppe Pettinato, Wendy S Vanden Berg-Foels, Ning Zhang, Xuejun Wen |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4217711?pdf=render |
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