Genetic diversity of Plantago ovata Forsk. through RAPD markers

• Main Author: Ashish G Vala1*, R.S.Fougat1 and G.C.Jadeja
• Format: Article
• Language: English
• Published: Indian Society of Plant Breeders 2011-12-01
• Series: Electronic Journal of Plant Breeding
• Subjects: Plantago ovata; genetic diversity; RAPD; sex-morphotypes
• Online Access: https://sites.google.com/site/ejpb2011/vol-2-4/Vol-2-4-592-596.pdf?attredirects=0

Genetic variability of 15 sets of Plantago ovata Forsk. studied using 11 arbitrary oligonucleotide primers. Among the 90 DNAfragments produced 71 fragments were found to be polymorphic. The mean number of polymorphic bands per primer among 15Plantago ovata genotypes was 6.45 . The higher polymorphism (90.00 %) was exhibited by primer OPF-17, while the lowerpolymorphism (60.00 %) was detected by OPF-2. The genetic similarity matrix from RAPD data for 15 genotypes was calculatedbased on Jaccard’s coefficients of similarity ranged from 0.45 to 0.80. UPGMA cluster analysis reveals that the 15 genotypeswere clustered in to three clusters. Genetically distinct genotypes identified using RAPD markers could be potential sources ofgermplasm for Isabgol improvement.