Flow cytometry analysis of different T cell markers in mastitic buffalo milk before and after treatment with Homeopathic medicine

• Main Authors: R. Sharma, A. Sharma, Y. Singh Rana, T. Khemani
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2010-02-01
• Series: Italian Journal of Animal Science
• Subjects: Flow cytometry, BoCD4+ T Lymphocyte, BoCD8+ T Lymphocyte, Mastacure
• Online Access: http://www.aspajournal.it/index.php/ijas/article/view/1171

The population of different T cell markers viz. BoCD4+, BoCD8+ and BoWC1+ cells were analyzed using monoclonal antibodies (MAb) against T cell markers by Flowcytometric analysis. There was significant increase in BoCD4+ and BoWC1+ T cells compared to healthy buffaloes (P<0.05). An increase was also recorded in BoCD8+ cells of mastitic buffaloes as compared to normal buffaloes but this elevation was not significant (P>0.05). After the start of homeopathic treatment there was a gradual decrease in BoCD8+ T cells on day 5 and 10. However, on day 20 there was an increase in this cell subpopulation, which was significant. There was significant increase in ratio of BoCD4+: BoCD8+ T lymphocytes on day 5 and day 10 post treatments indicating an up regulation of BoCD4+. On day 20 there was an apparent reduction in mean ratio of BoCD4+: BoCD8+ T lymphocytes but it remained more than one which is indicative of active immune response.