| Summary: | Objective To investigate the expression of proline-rich protein 11 (PRR11) in colorectal cancer and its role in regulating the invasion and metastasis of colorectal cancer. Methods The expression of PRR11 was detected using immunohistochemical SP method in 136 specimens of colorectal cancer tissues and 68 adjacent tissues collected from Jiangjin District Central Hospital between January, 2015 and March, 2018. We also examined the expression of PRR11 and epithelial-mesenchymal transition (EMT)-related signal molecules in 3 colorectal cancer cell lines (SW480, HT29 and HCT116) and normal human rectal epithelial cells (HCoEpiC) using Western blotting, RT-PCR and immunofluorescence assay; cell scratch assay and Transwell assay were used to compare the migration and invasion abilities of the colorectal cancer cells. Results The positive expression rate of PRR11 was significantly higher in colorectal cancer tissue than in the adjacent tissues (60.29% vs 1.47%, P < 0.01). PRR11 expression was significantly correlated with tumor differentiation, distant metastasis, tumor size and tumor staging (P < 0.05). Compared with normal colorectal epithelial cells, the colorectal cancer cell lines expressed high levels of PRR11. Overexpression of PRR11 in HT29 cells significantly enhanced the cell migration and invasion ability, caused obvious β-catenin nuclear translocation, significantly up-regulated the expression of β-catenin and vimentin, and decreased the expression of E-cadherin (P < 0.05). Silencing PRR11 significantly lowered the migration and invasion ability of HT29 cells, decreased the expression of β-catenin and vimentin, and increased the expression of E-cadherin (P < 0.05). Conclusion PRR11 is highly expressed in colorectal cancer to promote tumor invasion and metastasis possibly by promoting the nuclear translocation of β-catenin via activating the Wnt/β-catenin signaling pathway.
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