Development and mapping of SSR markers linked to resistance-gene homologue clusters in common bean

• Main Authors: Luz Nayibe Garzon, Matthew Wohlgemuth Blair
• Format: Article
• Language: English
• Published: KeAi Communications Co., Ltd. 2014-08-01
• Series: Crop Journal
• Subjects: Bacterial artificial chromosome (BAC) clone end sequences (BES); Simple sequence repeats (SSRs); Plant disease resistance (R) genes; Nucleotide binding site targeted sequencing; Resistance gene analogs
• Online Access: http://www.sciencedirect.com/science/article/pii/S2214514114000324

Common bean is an important but often a disease-susceptible legume crop of temperate, subtropical and tropical regions worldwide. The crop is affected by bacterial, fungal and viral pathogens. The strategy of resistance-gene homologue (RGH) cloning has proven to be an efficient tool for identifying markers and R (resistance) genes associated with resistances to diseases. Microsatellite or SSR markers can be identified by physical association with RGH clones on large-insert DNA clones such as bacterial artificial chromosomes (BACs). Our objectives in this work were to identify RGH-SSR in a BAC library from the Andean genotype G19833 and to test and map any polymorphic markers to identify associations with known positions of disease resistance genes. We developed a set of specific probes designed for clades of common bean RGH genes and then identified positive BAC clones and developed microsatellites from BACs having SSR loci in their end sequences. A total of 629 new RGH-SSRs were identified and named BMr (bean microsatellite RGH-associated markers). A subset of these markers was screened for detecting polymorphism in the genetic mapping population DOR364 × G19833. A genetic map was constructed with a total of 264 markers, among which were 80 RGH loci anchored to single-copy RFLP and SSR markers. Clusters of RGH-SSRs were observed on most of the linkage groups of common bean and in positions associated with R-genes and QTL. The use of these new markers to select for disease resistance is discussed.