Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask
The Sensing Cell Culture Flask (SCCF) is a cell culture monitoring system accessing the cellular microenvironment in 2D cell culture using electrochemical microsensors. The system is based on microfabricated sensor chips embedded in standard cell culture flasks. Ideally, the sensor chips could be eq...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-04-01
|
Series: | Biosensors |
Subjects: | |
Online Access: | http://www.mdpi.com/2079-6374/8/2/44 |
id |
doaj-cce9f29ab5cd49d1a2be7e08b7f02c1d |
---|---|
record_format |
Article |
spelling |
doaj-cce9f29ab5cd49d1a2be7e08b7f02c1d2020-11-24T23:26:27ZengMDPI AGBiosensors2079-63742018-04-01824410.3390/bios8020044bios8020044Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture FlaskJochen Kieninger0Yaara Tamari1Barbara Enderle2Gerhard Jobst3Joe A. Sandvik4Erik O. Pettersen5Gerald A. Urban6Laboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, GermanyLaboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, GermanyLaboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, GermanyJobst Technologies GmbH, Engesserstraße 4b, D-79108 Freiburg, GermanyDepartment of Physics, University of Oslo, 1048 Blindern, N-0316 Oslo, NorwayDepartment of Physics, University of Oslo, 1048 Blindern, N-0316 Oslo, NorwayLaboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, GermanyThe Sensing Cell Culture Flask (SCCF) is a cell culture monitoring system accessing the cellular microenvironment in 2D cell culture using electrochemical microsensors. The system is based on microfabricated sensor chips embedded in standard cell culture flasks. Ideally, the sensor chips could be equipped with any electrochemical sensor. Its transparency allows optical inspection of the cells during measurement. The surface of the sensor chip is in-plane with the flask surface allowing undisturbed cell growth on the sensor chip. A custom developed rack system allows easy usage of multiple flasks in parallel within an incubator. The presented data demonstrates the application of the SCCF with brain tumor (T98G) and breast cancer (T-47D) cells. Amperometric oxygen sensors were used to monitor cellular respiration with different incubation conditions. Cellular acidification was accessed with potentiometric pH sensors using electrodeposited iridium oxide films. The system itself provides the foundation for electrochemical monitoring systems in 3D cell culture.http://www.mdpi.com/2079-6374/8/2/44cell culture monitoringmicrosensoroxygenpHhypoxia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jochen Kieninger Yaara Tamari Barbara Enderle Gerhard Jobst Joe A. Sandvik Erik O. Pettersen Gerald A. Urban |
spellingShingle |
Jochen Kieninger Yaara Tamari Barbara Enderle Gerhard Jobst Joe A. Sandvik Erik O. Pettersen Gerald A. Urban Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask Biosensors cell culture monitoring microsensor oxygen pH hypoxia |
author_facet |
Jochen Kieninger Yaara Tamari Barbara Enderle Gerhard Jobst Joe A. Sandvik Erik O. Pettersen Gerald A. Urban |
author_sort |
Jochen Kieninger |
title |
Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask |
title_short |
Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask |
title_full |
Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask |
title_fullStr |
Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask |
title_full_unstemmed |
Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask |
title_sort |
sensor access to the cellular microenvironment using the sensing cell culture flask |
publisher |
MDPI AG |
series |
Biosensors |
issn |
2079-6374 |
publishDate |
2018-04-01 |
description |
The Sensing Cell Culture Flask (SCCF) is a cell culture monitoring system accessing the cellular microenvironment in 2D cell culture using electrochemical microsensors. The system is based on microfabricated sensor chips embedded in standard cell culture flasks. Ideally, the sensor chips could be equipped with any electrochemical sensor. Its transparency allows optical inspection of the cells during measurement. The surface of the sensor chip is in-plane with the flask surface allowing undisturbed cell growth on the sensor chip. A custom developed rack system allows easy usage of multiple flasks in parallel within an incubator. The presented data demonstrates the application of the SCCF with brain tumor (T98G) and breast cancer (T-47D) cells. Amperometric oxygen sensors were used to monitor cellular respiration with different incubation conditions. Cellular acidification was accessed with potentiometric pH sensors using electrodeposited iridium oxide films. The system itself provides the foundation for electrochemical monitoring systems in 3D cell culture. |
topic |
cell culture monitoring microsensor oxygen pH hypoxia |
url |
http://www.mdpi.com/2079-6374/8/2/44 |
work_keys_str_mv |
AT jochenkieninger sensoraccesstothecellularmicroenvironmentusingthesensingcellcultureflask AT yaaratamari sensoraccesstothecellularmicroenvironmentusingthesensingcellcultureflask AT barbaraenderle sensoraccesstothecellularmicroenvironmentusingthesensingcellcultureflask AT gerhardjobst sensoraccesstothecellularmicroenvironmentusingthesensingcellcultureflask AT joeasandvik sensoraccesstothecellularmicroenvironmentusingthesensingcellcultureflask AT erikopettersen sensoraccesstothecellularmicroenvironmentusingthesensingcellcultureflask AT geraldaurban sensoraccesstothecellularmicroenvironmentusingthesensingcellcultureflask |
_version_ |
1725555029674295296 |