Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask

The Sensing Cell Culture Flask (SCCF) is a cell culture monitoring system accessing the cellular microenvironment in 2D cell culture using electrochemical microsensors. The system is based on microfabricated sensor chips embedded in standard cell culture flasks. Ideally, the sensor chips could be eq...

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Main Authors: Jochen Kieninger, Yaara Tamari, Barbara Enderle, Gerhard Jobst, Joe A. Sandvik, Erik O. Pettersen, Gerald A. Urban
Format: Article
Language:English
Published: MDPI AG 2018-04-01
Series:Biosensors
Subjects:
pH
Online Access:http://www.mdpi.com/2079-6374/8/2/44
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spelling doaj-cce9f29ab5cd49d1a2be7e08b7f02c1d2020-11-24T23:26:27ZengMDPI AGBiosensors2079-63742018-04-01824410.3390/bios8020044bios8020044Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture FlaskJochen Kieninger0Yaara Tamari1Barbara Enderle2Gerhard Jobst3Joe A. Sandvik4Erik O. Pettersen5Gerald A. Urban6Laboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, GermanyLaboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, GermanyLaboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, GermanyJobst Technologies GmbH, Engesserstraße 4b, D-79108 Freiburg, GermanyDepartment of Physics, University of Oslo, 1048 Blindern, N-0316 Oslo, NorwayDepartment of Physics, University of Oslo, 1048 Blindern, N-0316 Oslo, NorwayLaboratory for Sensors, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Köhler Allee 103, D-79110 Freiburg, GermanyThe Sensing Cell Culture Flask (SCCF) is a cell culture monitoring system accessing the cellular microenvironment in 2D cell culture using electrochemical microsensors. The system is based on microfabricated sensor chips embedded in standard cell culture flasks. Ideally, the sensor chips could be equipped with any electrochemical sensor. Its transparency allows optical inspection of the cells during measurement. The surface of the sensor chip is in-plane with the flask surface allowing undisturbed cell growth on the sensor chip. A custom developed rack system allows easy usage of multiple flasks in parallel within an incubator. The presented data demonstrates the application of the SCCF with brain tumor (T98G) and breast cancer (T-47D) cells. Amperometric oxygen sensors were used to monitor cellular respiration with different incubation conditions. Cellular acidification was accessed with potentiometric pH sensors using electrodeposited iridium oxide films. The system itself provides the foundation for electrochemical monitoring systems in 3D cell culture.http://www.mdpi.com/2079-6374/8/2/44cell culture monitoringmicrosensoroxygenpHhypoxia
collection DOAJ
language English
format Article
sources DOAJ
author Jochen Kieninger
Yaara Tamari
Barbara Enderle
Gerhard Jobst
Joe A. Sandvik
Erik O. Pettersen
Gerald A. Urban
spellingShingle Jochen Kieninger
Yaara Tamari
Barbara Enderle
Gerhard Jobst
Joe A. Sandvik
Erik O. Pettersen
Gerald A. Urban
Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask
Biosensors
cell culture monitoring
microsensor
oxygen
pH
hypoxia
author_facet Jochen Kieninger
Yaara Tamari
Barbara Enderle
Gerhard Jobst
Joe A. Sandvik
Erik O. Pettersen
Gerald A. Urban
author_sort Jochen Kieninger
title Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask
title_short Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask
title_full Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask
title_fullStr Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask
title_full_unstemmed Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask
title_sort sensor access to the cellular microenvironment using the sensing cell culture flask
publisher MDPI AG
series Biosensors
issn 2079-6374
publishDate 2018-04-01
description The Sensing Cell Culture Flask (SCCF) is a cell culture monitoring system accessing the cellular microenvironment in 2D cell culture using electrochemical microsensors. The system is based on microfabricated sensor chips embedded in standard cell culture flasks. Ideally, the sensor chips could be equipped with any electrochemical sensor. Its transparency allows optical inspection of the cells during measurement. The surface of the sensor chip is in-plane with the flask surface allowing undisturbed cell growth on the sensor chip. A custom developed rack system allows easy usage of multiple flasks in parallel within an incubator. The presented data demonstrates the application of the SCCF with brain tumor (T98G) and breast cancer (T-47D) cells. Amperometric oxygen sensors were used to monitor cellular respiration with different incubation conditions. Cellular acidification was accessed with potentiometric pH sensors using electrodeposited iridium oxide films. The system itself provides the foundation for electrochemical monitoring systems in 3D cell culture.
topic cell culture monitoring
microsensor
oxygen
pH
hypoxia
url http://www.mdpi.com/2079-6374/8/2/44
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