Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae)
The first and second internal transcribed spacer regions (ITS1 and ITS2) of the ribosomal DNA of Biomphalaria tenagophila complex (B. tenagophila, B. occidentalis, and B. t. guaibensis) were sequenced and compared. The alignment lengths of these regions were about 655 bp and 481 bp, respectively. Ph...
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Instituto Oswaldo Cruz, Ministério da Saúde
2004-03-01
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doaj-cca2b115d55a4deeab4f499fb8d8ccf32020-11-24T21:13:26ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80602004-03-0199215315810.1590/S0074-02762004000200007Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae)Teofânia HDA VidigalLinus SpatzJessica C KissingerRodrigo AF RedondoEdina CR PiresAndrew JG SimpsonOmar S CarvalhoThe first and second internal transcribed spacer regions (ITS1 and ITS2) of the ribosomal DNA of Biomphalaria tenagophila complex (B. tenagophila, B. occidentalis, and B. t. guaibensis) were sequenced and compared. The alignment lengths of these regions were about 655 bp and 481 bp, respectively. Phylogenetic relationships among the Biomphalaria species were inferred by Maximum Parsimony and Neighbor-joining methods. The phylogenetic trees produced, in most of the cases, were in accordance with morphological systematics and other molecular data previously obtained by polymerase chain reaction and restriction fragment length polymorphism analysis. The present results provide support for the proposal that B. tenagophila represents a complex comprising B. tenagophila, B. occidentalis and B. t. guaibensis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000200007Biomphalariasnailsribosomal DNAinternal transcribed spacerpolymerase chain reaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Teofânia HDA Vidigal Linus Spatz Jessica C Kissinger Rodrigo AF Redondo Edina CR Pires Andrew JG Simpson Omar S Carvalho |
spellingShingle |
Teofânia HDA Vidigal Linus Spatz Jessica C Kissinger Rodrigo AF Redondo Edina CR Pires Andrew JG Simpson Omar S Carvalho Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae) Memórias do Instituto Oswaldo Cruz. Biomphalaria snails ribosomal DNA internal transcribed spacer polymerase chain reaction |
author_facet |
Teofânia HDA Vidigal Linus Spatz Jessica C Kissinger Rodrigo AF Redondo Edina CR Pires Andrew JG Simpson Omar S Carvalho |
author_sort |
Teofânia HDA Vidigal |
title |
Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae) |
title_short |
Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae) |
title_full |
Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae) |
title_fullStr |
Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae) |
title_full_unstemmed |
Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae) |
title_sort |
analysis of the first and second internal transcribed spacer sequences of the ribosomal dna in biomphalaria tenagophila complex (mollusca: planorbidae) |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
2004-03-01 |
description |
The first and second internal transcribed spacer regions (ITS1 and ITS2) of the ribosomal DNA of Biomphalaria tenagophila complex (B. tenagophila, B. occidentalis, and B. t. guaibensis) were sequenced and compared. The alignment lengths of these regions were about 655 bp and 481 bp, respectively. Phylogenetic relationships among the Biomphalaria species were inferred by Maximum Parsimony and Neighbor-joining methods. The phylogenetic trees produced, in most of the cases, were in accordance with morphological systematics and other molecular data previously obtained by polymerase chain reaction and restriction fragment length polymorphism analysis. The present results provide support for the proposal that B. tenagophila represents a complex comprising B. tenagophila, B. occidentalis and B. t. guaibensis. |
topic |
Biomphalaria snails ribosomal DNA internal transcribed spacer polymerase chain reaction |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000200007 |
work_keys_str_mv |
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