Landscapes of Protein Posttranslational Modifications of African Trypanosoma Parasites
Summary: Proteins of all living cells undergo a myriad of post-translational modifications (PTMs) that are critical to multifarious life processes. In this study, we describe the first comprehensive multiple PTM-omics atlas in parallel with quantitative proteome analyses of two representative specie...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-05-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004220302595 |
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doaj-cc93c1359fd04d33b61dd9a83559d388 |
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| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Naiwen Zhang Ning Jiang Kai Zhang Lili Zheng Di Zhang Xiaoyu Sang Ying Feng Ran Chen Na Yang Xinyi Wang Zhongyi Cheng Xun Suo Zhaorong Lun Qijun Chen |
| spellingShingle |
Naiwen Zhang Ning Jiang Kai Zhang Lili Zheng Di Zhang Xiaoyu Sang Ying Feng Ran Chen Na Yang Xinyi Wang Zhongyi Cheng Xun Suo Zhaorong Lun Qijun Chen Landscapes of Protein Posttranslational Modifications of African Trypanosoma Parasites iScience Proteomics Metabolomics |
| author_facet |
Naiwen Zhang Ning Jiang Kai Zhang Lili Zheng Di Zhang Xiaoyu Sang Ying Feng Ran Chen Na Yang Xinyi Wang Zhongyi Cheng Xun Suo Zhaorong Lun Qijun Chen |
| author_sort |
Naiwen Zhang |
| title |
Landscapes of Protein Posttranslational Modifications of African Trypanosoma Parasites |
| title_short |
Landscapes of Protein Posttranslational Modifications of African Trypanosoma Parasites |
| title_full |
Landscapes of Protein Posttranslational Modifications of African Trypanosoma Parasites |
| title_fullStr |
Landscapes of Protein Posttranslational Modifications of African Trypanosoma Parasites |
| title_full_unstemmed |
Landscapes of Protein Posttranslational Modifications of African Trypanosoma Parasites |
| title_sort |
landscapes of protein posttranslational modifications of african trypanosoma parasites |
| publisher |
Elsevier |
| series |
iScience |
| issn |
2589-0042 |
| publishDate |
2020-05-01 |
| description |
Summary: Proteins of all living cells undergo a myriad of post-translational modifications (PTMs) that are critical to multifarious life processes. In this study, we describe the first comprehensive multiple PTM-omics atlas in parallel with quantitative proteome analyses of two representative species of African trypanosomes, Trypanosoma brucei and Trypanosoma evansi. Ten PTM types with approximately 40,000 modified sites and 150 histone marks with a fine map on each protein of the two African trypanosomes were accomplished. The two biologically different trypanosomal species displayed distinct PTM-omic features, regulation pathways, and networks. Modifications in the proteins involved in the redox system were mainly upregulated in T. brucei, whereas proteins associated with motility were predominantly modified in T. evansi. The establishment of a database of multiple PTMs in the two parasites provides us with a deep insight into the biological mechanisms that underpin life processes in trypanosomes with different life cycles. |
| topic |
Proteomics Metabolomics |
| url |
http://www.sciencedirect.com/science/article/pii/S2589004220302595 |
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doaj-cc93c1359fd04d33b61dd9a83559d3882020-11-25T03:24:05ZengElsevieriScience2589-00422020-05-01235Landscapes of Protein Posttranslational Modifications of African Trypanosoma ParasitesNaiwen Zhang0Ning Jiang1Kai Zhang2Lili Zheng3Di Zhang4Xiaoyu Sang5Ying Feng6Ran Chen7Na Yang8Xinyi Wang9Zhongyi Cheng10Xun Suo11Zhaorong Lun12Qijun Chen13Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China; The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China; The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China; The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China; The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China; The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China; The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China; The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, ChinaCollege of Basic Sciences, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, ChinaJingjie PTM Biolab (Hangzhou) Co. Ltd, Hangzhou 310018, ChinaNational Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China; The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang 110866, China; Corresponding authorSummary: Proteins of all living cells undergo a myriad of post-translational modifications (PTMs) that are critical to multifarious life processes. In this study, we describe the first comprehensive multiple PTM-omics atlas in parallel with quantitative proteome analyses of two representative species of African trypanosomes, Trypanosoma brucei and Trypanosoma evansi. Ten PTM types with approximately 40,000 modified sites and 150 histone marks with a fine map on each protein of the two African trypanosomes were accomplished. The two biologically different trypanosomal species displayed distinct PTM-omic features, regulation pathways, and networks. Modifications in the proteins involved in the redox system were mainly upregulated in T. brucei, whereas proteins associated with motility were predominantly modified in T. evansi. The establishment of a database of multiple PTMs in the two parasites provides us with a deep insight into the biological mechanisms that underpin life processes in trypanosomes with different life cycles.http://www.sciencedirect.com/science/article/pii/S2589004220302595ProteomicsMetabolomics |