Single-Cell Imaging of Metastatic Potential of Cancer Cells

• Main Authors: Krishna Midde, Nina Sun, Cristina Rohena, Linda Joosen, Harsharan Dhillon, Pradipta Ghosh
• Format: Article
• Language: English
• Published: Elsevier 2018-12-01
• Series: iScience
• Online Access: http://www.sciencedirect.com/science/article/pii/S2589004218302128

Summary: Molecular imaging of metastatic “potential” is an unvanquished challenge. To engineer biosensors that can detect and measure the metastatic “potential” of single living cancer cells, we carried out a comprehensive analysis of the pan-cancer phosphoproteome to search for actin remodelers required for cell migration, which are enriched in cancers but excluded in normal cells. Only one phosphoprotein emerged, tyr-phosphorylated CCDC88A (GIV/Girdin), a bona fide metastasis-related protein across a variety of solid tumors. We designed multi-modular biosensors that are partly derived from GIV, and because GIV integrates prometastatic signaling by multiple oncogenic receptors, we named them “‘integrators of metastatic potential (IMP).” IMPs captured the heterogeneity of metastatic potential within primary lung and breast tumors at steady state, detected those few cells that have acquired the highest metastatic potential, and tracked their enrichment during metastasis. These findings provide proof of concept that IMPs can measure the diversity and plasticity of metastatic potential of tumor cells in a sensitive and unbiased way. : Optical Imaging; Protein Physics; Cancer Subject Areas: Optical Imaging, Protein Physics, Cancer