Comparative In silico Study of Sex-Determining Region Y (SRY) Protein Sequences Involved in Sex-Determining

• Main Authors: Masoume Vakili Azghandi, Mohammadreza Nasiri, Ali Shamsa, Mohsen Jalali, Mohammad Mahdi Shariati
• Format: Article
• Language: English
• Published: Varastegan Institute for Medical Sciences 2016-05-01
• Series: Reports of Biochemistry and Molecular Biology
• Subjects: Bioinformatics analysis; SRY gene; Phylogeny
• Online Access: http://rbmb.net/article-1-74-en.pdf

Background: The SRY gene (SRY) provides instructions for making a transcription factor called the sex-determining region Y protein. The sex-determining region Y protein causes a fetus to develop as a male. In this study, SRY of 15 spices included of human, chimpanzee, dog, pig, rat, cattle, buffalo, goat, sheep, horse, zebra, frog, urial, dolphin and killer whale were used for determine of bioinformatic differences. Methods: Nucleotide sequences of SRY were retrieved from the NCBI databank. Bioinformatic analysis of SRY is done by CLC Main Workbench version 5.5 and ClustalW (http:/www.ebi.ac.uk/clustalw/) and MEGA6 softwares. Results: The multiple sequence alignment results indicated that SRY protein sequences from Orcinus orca (killer whale) and Tursiopsaduncus (dolphin) have least genetic distance of 0.33 in these 15 species and are 99.67% identical at the amino acid level. Homosapiens and Pantroglodytes (chimpanzee) have the next lowest genetic distance of 1.35 and are 98.65% identical at the amino acid level. Conclusion: These findings indicate that the SRY proteins are conserved in the 15 species, and their evolutionary relationships are similar.