A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice

A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice

Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal–oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector f...

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Bibliographic Details
Main Authors: Kui Xiang, Yuri Kusov, Guan Ying, Wang Yan, Yi Shan, Wu Jinyuan, Yin Na, Zhou Yan, Li Hongjun, Sun Maosheng
Format: Article
Language:English
Published: MDPI AG 2017-09-01
Series:Viruses
Subjects:
combined vaccine
hepatitis A
hepatitis E
virus
vector
neutralization epitope
Online Access:https://www.mdpi.com/1999-4915/9/9/260
Description
Summary:Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal–oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459–606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.
ISSN:1999-4915

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