Experience of Application of the ELISA Method for Detection of Antibodies to Ebola Virus during the SAET Team Work in the Republic of Guinea

• Main Authors: S. A. P’yankov, O. V. P’yankov, E. V. Naydenova, A. P. Agafonov, M. Y. Boiro, V. V. Solodkiy, A. V. Zaykovskaya, N. L. Maksimov, S. S. Marennikova, E. F. Bocharov, V. I. Ofitserov, A. A. Lopatin, S. A. Shcherbakova, Yu. V. Demina, V. V. Kutyrev, V. N. Mikheev
• Format: Article
• Language: Russian
• Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2016-09-01
• Series: Проблемы особо опасных инфекций
• Subjects: ebola virus; laboratory diagnostics; elisa; dynamics of antibodies titer increment
• Online Access: https://journal.microbe.ru/jour/article/view/330

The aim of the work was to develop a kit for detection of IgM and IgG antibodies to Ebola virus in ELISA.Materials and methods. Ebola virus strain Zaire H.sapiens-wt/GIN/2015/Kalidie-Kindia-1022 grown on cell culture was used as antigen.Results and conclusions. The kit was used by the Rospotrebnadzor SAET team in its work in Guinea while investigating cases during Ebola fever epidemics. It was established that specific IgG antibodies persisted in Ebola fever survivors for 2 years. Application of this kit in the laboratory diagnostics permits ELISA to become the main and confirmatory laboratory method of Ebola fever detection.