Mitochondrial activity of cancer and normal mesenchymal stem cells in vitro cultured in medium with different deuterium content
We showed that cancer and normal mesenchymal stem/stromal (MSC) cells in vitro in a deuterated growth medium show a decrease of mitochondrial activity (MA), while in a deuterium-depleted medium an increase. This was established using mitotracker and rhodamine 123, and was also confirmed by expressio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2020-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/full_html/2020/06/bioconf_icli2020_02005/bioconf_icli2020_02005.html |
| Summary: | We showed that cancer and normal mesenchymal stem/stromal (MSC) cells in vitro in a deuterated growth medium show a decrease of mitochondrial activity (MA), while in a deuterium-depleted medium an increase. This was established using mitotracker and rhodamine 123, and was also confirmed by expression of the UCP1 gene. The correlation dependence of mitochondrial activity in a medium with a changed ratio of deuterium/protium (D/H) and supramolecular structures was established, using the laser diffraction method. Density inhomogeneities in the deuterated medium are noted to be large, and in the deuterium-depleted medium small, in comparison with the control. |
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| ISSN: | 2117-4458 |