A new heterotropic vascularized model of total urinary bladder transplantation in a rat model

Abstract This study developed a new procedure of urinary bladder transplantation on a rat model (n = 40). Heterotopic urinary bladder transplantation (n = 10) in the right groin vessels was performed. Direct urinary bladder examination, microangiography, histological analysis, and India ink injectio...

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Main Authors: Arkadiusz Jundziłł, Henryk Witmanowski, Ewa Żary-Sikorska, Jan Adamowicz, Magdalena Bodnar, Andrzej Marszałek, Tomasz Kloskowski, Kaja Męcińska-Jundziłł, Maciej Gagat, Natalia Siedlecka, Tomasz Drewa, Marta Pokrywczyńska
Format: Article
Language:English
Published: Nature Publishing Group 2021-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-83128-w
Description
Summary:Abstract This study developed a new procedure of urinary bladder transplantation on a rat model (n = 40). Heterotopic urinary bladder transplantation (n = 10) in the right groin vessels was performed. Direct urinary bladder examination, microangiography, histological analysis, and India ink injection were performed to evaluate the proposed method's functionality. Observation time was four weeks. One week after the procedure, the graft survival rate was 80%, two urinary bladders were lost due to anastomosis failure. The rest of the grafts survived two weeks without any complications. Lack of transitional epithelium or smooth muscle layer loss and lack of inflammatory process development were observed. This study was performed in order to obtain the necessary knowledge about urinary bladder transplantation. The proposed technique offers a new approach to the existing orthotropic models.
ISSN:2045-2322