Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells

In a previous study, we demonstrated the anti-inflammatory activity of the essential oil extracted from Korean pine (<i>Pinus koraiensis</i>, Sieb. et Zucc.) wood. This study aims to investigate the active anti-inflammatory constituents of <i>P. koraiensis</i> oil. The essent...

Full description

Bibliographic Details
Main Authors: Ji-Yoon Yang, Won-Sil Choi, Ki-Joong Kim, Chang-Deuk Eom, Mi-Jin Park
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:Biomolecules
Subjects:
Online Access:https://www.mdpi.com/2218-273X/11/6/817
id doaj-cbfb1d4f92ef40fda3d790ea980cef30
record_format Article
spelling doaj-cbfb1d4f92ef40fda3d790ea980cef302021-06-01T01:43:42ZengMDPI AGBiomolecules2218-273X2021-05-011181781710.3390/biom11060817Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 CellsJi-Yoon Yang0Won-Sil Choi1Ki-Joong Kim2Chang-Deuk Eom3Mi-Jin Park4Division of Forest Industrial Materials, Department of Forest Products and Industry, National Institute of Forest Science, Seoul 02455, KoreaNational Instrumentation Center for Environmental Management, Seoul National University, Seoul 08826, KoreaDivision of Life Sciences, School of Life Sciences, Korea University, Seoul 02841, KoreaDivision of Wood Industry, Department of Forest Products and Industry, National Institute of Forest Science, Seoul 02455, KoreaDivision of Forest Industrial Materials, Department of Forest Products and Industry, National Institute of Forest Science, Seoul 02455, KoreaIn a previous study, we demonstrated the anti-inflammatory activity of the essential oil extracted from Korean pine (<i>Pinus koraiensis</i>, Sieb. et Zucc.) wood. This study aims to investigate the active anti-inflammatory constituents of <i>P. koraiensis</i> oil. The essential oil was extracted from <i>P. koraiensis</i> wood by hydrodistillation and was divided into six fractions (A–F) through fractional distillation. Then, the anti-inflammatory activities of the fractions (A–F) were determined. Fractions A and F markedly downregulated the production of pro-inflammatory cytokines as well as the secretion of <i>β</i>-hexosaminidase in lipopolysaccharide (LPS)-stimulated RBL-2H3 cells. The main constituents of the active anti-inflammatory A and F fractions were (+)-<i>α</i>-pinene, (−)-<i>β</i>-pinene, (+)-<i>α</i>-terpineol, 3-carene, (+)-limonene, and longifolene. These six single compounds decreased the expression of inflammatory-related genes (i.e., IL-4 and IL-13) as well as the secretion of <i>β</i>-hexosaminidase in LPS-stimulated RBL-2H3 cells. (+)-<i>α</i>-Pinene, (−)-<i>β</i>-pinene, (+)-<i>α</i>-terpineol, and longifolene exhibited the strongest effects; these effects were comparable to those of the positive control (i.e., dexamethasone). The findings indicate that the interactions between these components exhibit potential for the management and/or treatment of inflammatory conditions as well as base structures for the development of novel anti-inflammatory drugs.https://www.mdpi.com/2218-273X/11/6/817<i>Pinus koraiensis</i>essential oilanti-inflammatoryactive compound<i>α</i>-pinene<i>β</i>-pinene
collection DOAJ
language English
format Article
sources DOAJ
author Ji-Yoon Yang
Won-Sil Choi
Ki-Joong Kim
Chang-Deuk Eom
Mi-Jin Park
spellingShingle Ji-Yoon Yang
Won-Sil Choi
Ki-Joong Kim
Chang-Deuk Eom
Mi-Jin Park
Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells
Biomolecules
<i>Pinus koraiensis</i>
essential oil
anti-inflammatory
active compound
<i>α</i>-pinene
<i>β</i>-pinene
author_facet Ji-Yoon Yang
Won-Sil Choi
Ki-Joong Kim
Chang-Deuk Eom
Mi-Jin Park
author_sort Ji-Yoon Yang
title Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells
title_short Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells
title_full Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells
title_fullStr Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells
title_full_unstemmed Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells
title_sort investigation of active anti-inflammatory constituents of essential oil from <i>pinus koraiensis</i> (sieb. et zucc.) wood in lps-stimulated rbl-2h3 cells
publisher MDPI AG
series Biomolecules
issn 2218-273X
publishDate 2021-05-01
description In a previous study, we demonstrated the anti-inflammatory activity of the essential oil extracted from Korean pine (<i>Pinus koraiensis</i>, Sieb. et Zucc.) wood. This study aims to investigate the active anti-inflammatory constituents of <i>P. koraiensis</i> oil. The essential oil was extracted from <i>P. koraiensis</i> wood by hydrodistillation and was divided into six fractions (A–F) through fractional distillation. Then, the anti-inflammatory activities of the fractions (A–F) were determined. Fractions A and F markedly downregulated the production of pro-inflammatory cytokines as well as the secretion of <i>β</i>-hexosaminidase in lipopolysaccharide (LPS)-stimulated RBL-2H3 cells. The main constituents of the active anti-inflammatory A and F fractions were (+)-<i>α</i>-pinene, (−)-<i>β</i>-pinene, (+)-<i>α</i>-terpineol, 3-carene, (+)-limonene, and longifolene. These six single compounds decreased the expression of inflammatory-related genes (i.e., IL-4 and IL-13) as well as the secretion of <i>β</i>-hexosaminidase in LPS-stimulated RBL-2H3 cells. (+)-<i>α</i>-Pinene, (−)-<i>β</i>-pinene, (+)-<i>α</i>-terpineol, and longifolene exhibited the strongest effects; these effects were comparable to those of the positive control (i.e., dexamethasone). The findings indicate that the interactions between these components exhibit potential for the management and/or treatment of inflammatory conditions as well as base structures for the development of novel anti-inflammatory drugs.
topic <i>Pinus koraiensis</i>
essential oil
anti-inflammatory
active compound
<i>α</i>-pinene
<i>β</i>-pinene
url https://www.mdpi.com/2218-273X/11/6/817
work_keys_str_mv AT jiyoonyang investigationofactiveantiinflammatoryconstituentsofessentialoilfromipinuskoraiensisisiebetzuccwoodinlpsstimulatedrbl2h3cells
AT wonsilchoi investigationofactiveantiinflammatoryconstituentsofessentialoilfromipinuskoraiensisisiebetzuccwoodinlpsstimulatedrbl2h3cells
AT kijoongkim investigationofactiveantiinflammatoryconstituentsofessentialoilfromipinuskoraiensisisiebetzuccwoodinlpsstimulatedrbl2h3cells
AT changdeukeom investigationofactiveantiinflammatoryconstituentsofessentialoilfromipinuskoraiensisisiebetzuccwoodinlpsstimulatedrbl2h3cells
AT mijinpark investigationofactiveantiinflammatoryconstituentsofessentialoilfromipinuskoraiensisisiebetzuccwoodinlpsstimulatedrbl2h3cells
_version_ 1721411725614383104