Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells
In a previous study, we demonstrated the anti-inflammatory activity of the essential oil extracted from Korean pine (<i>Pinus koraiensis</i>, Sieb. et Zucc.) wood. This study aims to investigate the active anti-inflammatory constituents of <i>P. koraiensis</i> oil. The essent...
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doaj-cbfb1d4f92ef40fda3d790ea980cef302021-06-01T01:43:42ZengMDPI AGBiomolecules2218-273X2021-05-011181781710.3390/biom11060817Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 CellsJi-Yoon Yang0Won-Sil Choi1Ki-Joong Kim2Chang-Deuk Eom3Mi-Jin Park4Division of Forest Industrial Materials, Department of Forest Products and Industry, National Institute of Forest Science, Seoul 02455, KoreaNational Instrumentation Center for Environmental Management, Seoul National University, Seoul 08826, KoreaDivision of Life Sciences, School of Life Sciences, Korea University, Seoul 02841, KoreaDivision of Wood Industry, Department of Forest Products and Industry, National Institute of Forest Science, Seoul 02455, KoreaDivision of Forest Industrial Materials, Department of Forest Products and Industry, National Institute of Forest Science, Seoul 02455, KoreaIn a previous study, we demonstrated the anti-inflammatory activity of the essential oil extracted from Korean pine (<i>Pinus koraiensis</i>, Sieb. et Zucc.) wood. This study aims to investigate the active anti-inflammatory constituents of <i>P. koraiensis</i> oil. The essential oil was extracted from <i>P. koraiensis</i> wood by hydrodistillation and was divided into six fractions (A–F) through fractional distillation. Then, the anti-inflammatory activities of the fractions (A–F) were determined. Fractions A and F markedly downregulated the production of pro-inflammatory cytokines as well as the secretion of <i>β</i>-hexosaminidase in lipopolysaccharide (LPS)-stimulated RBL-2H3 cells. The main constituents of the active anti-inflammatory A and F fractions were (+)-<i>α</i>-pinene, (−)-<i>β</i>-pinene, (+)-<i>α</i>-terpineol, 3-carene, (+)-limonene, and longifolene. These six single compounds decreased the expression of inflammatory-related genes (i.e., IL-4 and IL-13) as well as the secretion of <i>β</i>-hexosaminidase in LPS-stimulated RBL-2H3 cells. (+)-<i>α</i>-Pinene, (−)-<i>β</i>-pinene, (+)-<i>α</i>-terpineol, and longifolene exhibited the strongest effects; these effects were comparable to those of the positive control (i.e., dexamethasone). The findings indicate that the interactions between these components exhibit potential for the management and/or treatment of inflammatory conditions as well as base structures for the development of novel anti-inflammatory drugs.https://www.mdpi.com/2218-273X/11/6/817<i>Pinus koraiensis</i>essential oilanti-inflammatoryactive compound<i>α</i>-pinene<i>β</i>-pinene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ji-Yoon Yang Won-Sil Choi Ki-Joong Kim Chang-Deuk Eom Mi-Jin Park |
spellingShingle |
Ji-Yoon Yang Won-Sil Choi Ki-Joong Kim Chang-Deuk Eom Mi-Jin Park Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells Biomolecules <i>Pinus koraiensis</i> essential oil anti-inflammatory active compound <i>α</i>-pinene <i>β</i>-pinene |
author_facet |
Ji-Yoon Yang Won-Sil Choi Ki-Joong Kim Chang-Deuk Eom Mi-Jin Park |
author_sort |
Ji-Yoon Yang |
title |
Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells |
title_short |
Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells |
title_full |
Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells |
title_fullStr |
Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells |
title_full_unstemmed |
Investigation of Active Anti-Inflammatory Constituents of Essential Oil from <i>Pinus koraiensis</i> (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells |
title_sort |
investigation of active anti-inflammatory constituents of essential oil from <i>pinus koraiensis</i> (sieb. et zucc.) wood in lps-stimulated rbl-2h3 cells |
publisher |
MDPI AG |
series |
Biomolecules |
issn |
2218-273X |
publishDate |
2021-05-01 |
description |
In a previous study, we demonstrated the anti-inflammatory activity of the essential oil extracted from Korean pine (<i>Pinus koraiensis</i>, Sieb. et Zucc.) wood. This study aims to investigate the active anti-inflammatory constituents of <i>P. koraiensis</i> oil. The essential oil was extracted from <i>P. koraiensis</i> wood by hydrodistillation and was divided into six fractions (A–F) through fractional distillation. Then, the anti-inflammatory activities of the fractions (A–F) were determined. Fractions A and F markedly downregulated the production of pro-inflammatory cytokines as well as the secretion of <i>β</i>-hexosaminidase in lipopolysaccharide (LPS)-stimulated RBL-2H3 cells. The main constituents of the active anti-inflammatory A and F fractions were (+)-<i>α</i>-pinene, (−)-<i>β</i>-pinene, (+)-<i>α</i>-terpineol, 3-carene, (+)-limonene, and longifolene. These six single compounds decreased the expression of inflammatory-related genes (i.e., IL-4 and IL-13) as well as the secretion of <i>β</i>-hexosaminidase in LPS-stimulated RBL-2H3 cells. (+)-<i>α</i>-Pinene, (−)-<i>β</i>-pinene, (+)-<i>α</i>-terpineol, and longifolene exhibited the strongest effects; these effects were comparable to those of the positive control (i.e., dexamethasone). The findings indicate that the interactions between these components exhibit potential for the management and/or treatment of inflammatory conditions as well as base structures for the development of novel anti-inflammatory drugs. |
topic |
<i>Pinus koraiensis</i> essential oil anti-inflammatory active compound <i>α</i>-pinene <i>β</i>-pinene |
url |
https://www.mdpi.com/2218-273X/11/6/817 |
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