Identification and characterization of cryoprotective peptides extracted from silver carp (Hypophthalmichthys molitrix) hydrolysates

• Main Authors: Qingjing Liu, Yongle Liu, Jian Yu, Faxiang Wang, Xianghong Li
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2019-01-01
• Series: International Journal of Food Properties
• Subjects: silver carp hydrolysate; peptide; cryoprotective ability; surimi; identification
• Online Access: http://dx.doi.org/10.1080/10942912.2019.1622563

The cryoprotective effects on surimi matrix of hydrolysates with different hydrolyzing times prepared from silver carp using Protamex were compared with commercial sorbitol-sucrose cryoprotectants. Cryoprotective assays showed that FPH-30 (hydrolysate with 30 min hydrolyzing) was a more effective cryoprotectant than other hydrolysates, because the FPH-30 group displayed lower salt-soluble protein extractability loss (29.90 ± 0.84%), less actomyosin Ca2+-ATPase activity decrease (48.85 ± 2.56%) and unfrozen water content decrease (10.39 ± 0.63%) after six freeze-thaw cycles. To exploit industrial utilizations, FPH-30 was further purified with ultrafiltration membranes. The cryoprotective activity of the fraction (<3 kDa) was superior to the others by reducing the salt-soluble protein extractability loss (27.21 ± 0.98%) and impeding the actomyosin Ca2+-ATPase activity decrease (30.54 ± 1.21%). Mass spectrometry analysis showed that the fraction (<3 kDa) was mainly composed of three peptides of the sequences GVDNPGHPFIM(T) and IITNWDDMEK. The results clearly support that silver carp could be a potential source of cryoprotectant peptides for industrial applications.