A Transcriptomic and Epigenomic Comparison of Fetal and Adult Human Cardiac Fibroblasts Reveals Novel Key Transcription Factors in Adult Cardiac Fibroblasts
Cardiovascular disease remains the number one global cause of death and presents as multiple phenotypes in which the interplay between cardiomyocytes and cardiac fibroblasts (CFs) has become increasingly highlighted. Fetal and adult CFs influence neighboring cardiomyocytes in different ways. Thus fa...
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Elsevier
2016-12-01
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| Series: | JACC: Basic to Translational Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452302X16301140 |
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doaj-cbd8db2acc4245a7aaa97a5f73dec45f2020-11-24T20:55:54ZengElsevierJACC: Basic to Translational Science2452-302X2016-12-011759060210.1016/j.jacbts.2016.07.007A Transcriptomic and Epigenomic Comparison of Fetal and Adult Human Cardiac Fibroblasts Reveals Novel Key Transcription Factors in Adult Cardiac FibroblastsMalin K.B. Jonsson, PhD0Robin J.G. Hartman, MSc1Matthew Ackers-Johnson, PhD2Wilson L.W. Tan, BSc3Bing Lim, MD, PhD4Toon A.B. van Veen, PhD5Roger S. Foo, MD6Human Genetics, Genome Institute of Singapore, SingaporeHuman Genetics, Genome Institute of Singapore, SingaporeHuman Genetics, Genome Institute of Singapore, SingaporeHuman Genetics, Genome Institute of Singapore, SingaporeMerck Research Laboratories, Translational Medicine Research Centre, SingaporeDepartment of Medical Physiology, Division of Heart & Lungs, University Medical Center Utrecht, Utrecht, the NetherlandsHuman Genetics, Genome Institute of Singapore, SingaporeCardiovascular disease remains the number one global cause of death and presents as multiple phenotypes in which the interplay between cardiomyocytes and cardiac fibroblasts (CFs) has become increasingly highlighted. Fetal and adult CFs influence neighboring cardiomyocytes in different ways. Thus far, a detailed comparison between the two is lacking. Using a genome-wide approach, we identified and validated 2 crucial players for maintaining the adult primary human CF phenotype. Knockdown of these factors induced significant phenotypical changes, including senescence and reduced collagen gene expression. These may now represent novel therapeutic targets against deleterious functions of CFs in adult cardiovascular disease.http://www.sciencedirect.com/science/article/pii/S2452302X16301140cardiac fibroblastscollagenhistone methylationtranscriptome |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Malin K.B. Jonsson, PhD Robin J.G. Hartman, MSc Matthew Ackers-Johnson, PhD Wilson L.W. Tan, BSc Bing Lim, MD, PhD Toon A.B. van Veen, PhD Roger S. Foo, MD |
| spellingShingle |
Malin K.B. Jonsson, PhD Robin J.G. Hartman, MSc Matthew Ackers-Johnson, PhD Wilson L.W. Tan, BSc Bing Lim, MD, PhD Toon A.B. van Veen, PhD Roger S. Foo, MD A Transcriptomic and Epigenomic Comparison of Fetal and Adult Human Cardiac Fibroblasts Reveals Novel Key Transcription Factors in Adult Cardiac Fibroblasts JACC: Basic to Translational Science cardiac fibroblasts collagen histone methylation transcriptome |
| author_facet |
Malin K.B. Jonsson, PhD Robin J.G. Hartman, MSc Matthew Ackers-Johnson, PhD Wilson L.W. Tan, BSc Bing Lim, MD, PhD Toon A.B. van Veen, PhD Roger S. Foo, MD |
| author_sort |
Malin K.B. Jonsson, PhD |
| title |
A Transcriptomic and Epigenomic Comparison of Fetal and Adult Human Cardiac Fibroblasts Reveals Novel Key Transcription Factors in Adult Cardiac Fibroblasts |
| title_short |
A Transcriptomic and Epigenomic Comparison of Fetal and Adult Human Cardiac Fibroblasts Reveals Novel Key Transcription Factors in Adult Cardiac Fibroblasts |
| title_full |
A Transcriptomic and Epigenomic Comparison of Fetal and Adult Human Cardiac Fibroblasts Reveals Novel Key Transcription Factors in Adult Cardiac Fibroblasts |
| title_fullStr |
A Transcriptomic and Epigenomic Comparison of Fetal and Adult Human Cardiac Fibroblasts Reveals Novel Key Transcription Factors in Adult Cardiac Fibroblasts |
| title_full_unstemmed |
A Transcriptomic and Epigenomic Comparison of Fetal and Adult Human Cardiac Fibroblasts Reveals Novel Key Transcription Factors in Adult Cardiac Fibroblasts |
| title_sort |
transcriptomic and epigenomic comparison of fetal and adult human cardiac fibroblasts reveals novel key transcription factors in adult cardiac fibroblasts |
| publisher |
Elsevier |
| series |
JACC: Basic to Translational Science |
| issn |
2452-302X |
| publishDate |
2016-12-01 |
| description |
Cardiovascular disease remains the number one global cause of death and presents as multiple phenotypes in which the interplay between cardiomyocytes and cardiac fibroblasts (CFs) has become increasingly highlighted. Fetal and adult CFs influence neighboring cardiomyocytes in different ways. Thus far, a detailed comparison between the two is lacking. Using a genome-wide approach, we identified and validated 2 crucial players for maintaining the adult primary human CF phenotype. Knockdown of these factors induced significant phenotypical changes, including senescence and reduced collagen gene expression. These may now represent novel therapeutic targets against deleterious functions of CFs in adult cardiovascular disease. |
| topic |
cardiac fibroblasts collagen histone methylation transcriptome |
| url |
http://www.sciencedirect.com/science/article/pii/S2452302X16301140 |
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