A Simple Method for On-Gel Detection of Myrosinase Activity

A Simple Method for On-Gel Detection of Myrosinase Activity

Myrosinase is an enzyme present in many functional foods and spices, particularly in Cruciferous vegetables. It hydrolyses glucosinolates which thereafter rearrange into bioactive volatile constituents (isothiocyanates, nitriles). We aimed to develop a simple reversible method for on-gel detection o...

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Bibliographic Details
Main Authors: Sándor Gonda, Zsolt Szűcs, Tamás Plaszkó, Zoltán Cziáky, Attila Kiss-Szikszai, Gábor Vasas, Márta M-Hamvas
Format: Article
Language:English
Published: MDPI AG 2018-08-01
Series:Molecules
Subjects:
myrosinase
thioglucosidase
sulfatase
on-gel detection
desulfo-sinigrin
LC-ESI-MS
Online Access:http://www.mdpi.com/1420-3049/23/9/2204

Internet

http://www.mdpi.com/1420-3049/23/9/2204

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