Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual Maturity

Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual Maturity

Pigs share many anatomical and physiological features with humans, offering a unique and viable model for biomedical research. Although porcine female germline stem cells (FGSCs) were identified in the juvenile ovary, no reports described the isolation and purification of FGSCs from the pig at sexua...

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Main Authors: Lin Hou, Jian Wang, Xinyue Li, Hu Wang, Guishu Liu, Bo Xu, Xingxing Mei, Xiuguo Hua, Ji Wu
Format: Article
Language:English
Published: SAGE Publishing 2018-08-01
Series:Cell Transplantation
Online Access:https://doi.org/10.1177/0963689718784878
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spelling doaj-cbbdd177b072454bb56d4832a18c04cc2020-11-25T02:48:37ZengSAGE PublishingCell Transplantation0963-68971555-38922018-08-012710.1177/0963689718784878Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual MaturityLin Hou0Jian Wang1Xinyue Li2Hu Wang3Guishu Liu4Bo Xu5Xingxing Mei6Xiuguo Hua7Ji Wu8 Key Laboratory for the Genetics of Developmental, Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China Key Laboratory for the Genetics of Developmental, Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China Key Laboratory for the Genetics of Developmental, Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China Key Laboratory for the Genetics of Developmental, Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China The First People’s Hospital of Chenzhou, Chenzhou, Hunan, China Key Laboratory for the Genetics of Developmental, Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China Key Laboratory for the Genetics of Developmental, Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaPigs share many anatomical and physiological features with humans, offering a unique and viable model for biomedical research. Although porcine female germline stem cells (FGSCs) were identified in the juvenile ovary, no reports described the isolation and purification of FGSCs from the pig at sexual maturity. Here, we isolated, purified, and cultured FGSCs from porcine ovaries at sexual maturity. Furthermore, we established and characterized the porcine FGSC (pFGSC) lines. In addition, we found that pFGSC lines could differentiate into oocytes when injection into tissue grafts, including human ovarian tissues. The results show that FGSCs exist in ovaries of Banna mini-pigs at juvenile and sexually maturity. These findings have implications in animal biotechnology applications and regeneration medicine.https://doi.org/10.1177/0963689718784878
collection DOAJ
language English
format Article
sources DOAJ
author Lin Hou
Jian Wang
Xinyue Li
Hu Wang
Guishu Liu
Bo Xu
Xingxing Mei
Xiuguo Hua
Ji Wu
spellingShingle Lin Hou
Jian Wang
Xinyue Li
Hu Wang
Guishu Liu
Bo Xu
Xingxing Mei
Xiuguo Hua
Ji Wu
Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual Maturity
Cell Transplantation
author_facet Lin Hou
Jian Wang
Xinyue Li
Hu Wang
Guishu Liu
Bo Xu
Xingxing Mei
Xiuguo Hua
Ji Wu
author_sort Lin Hou
title Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual Maturity
title_short Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual Maturity
title_full Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual Maturity
title_fullStr Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual Maturity
title_full_unstemmed Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual Maturity
title_sort characteristics of female germline stem cells from porcine ovaries at sexual maturity
publisher SAGE Publishing
series Cell Transplantation
issn 0963-6897
1555-3892
publishDate 2018-08-01
description Pigs share many anatomical and physiological features with humans, offering a unique and viable model for biomedical research. Although porcine female germline stem cells (FGSCs) were identified in the juvenile ovary, no reports described the isolation and purification of FGSCs from the pig at sexual maturity. Here, we isolated, purified, and cultured FGSCs from porcine ovaries at sexual maturity. Furthermore, we established and characterized the porcine FGSC (pFGSC) lines. In addition, we found that pFGSC lines could differentiate into oocytes when injection into tissue grafts, including human ovarian tissues. The results show that FGSCs exist in ovaries of Banna mini-pigs at juvenile and sexually maturity. These findings have implications in animal biotechnology applications and regeneration medicine.
url https://doi.org/10.1177/0963689718784878
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