Ruminal ammonia concentration and fermentation kinetics of commercial herbal feed additives with amino acids

• Main Authors: Angélica Valeria Lorenzana Moreno, María Eugenia de la Torre Hernández, Augusto César Lizarazo Chaparro, Fernando Xicoténcatl Plata Pérez, Luis Alberto Miranda Romero, José Antonio Martínez García, Germán David Mendoza Martínez
• Format: Article
• Language: English
• Published: Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo 2020-11-01
• Series: Revista de la Facultad de Ciencias Agrarias
• Subjects: Allium sativa; Feed plant additive; Linum usitatissimum; lysine; methionine; ammonia nitrogen
• Online Access: http://200.12.138.7/ojs3/index.php/RFCA/article/view/4130
• ISSN: 0370-4661; 1853-8665

The objective of this study was to characterize the chemical composition of rumen fermentation while estimating it’s in vitro protein degradation (from ruminal ammonia concentration) and kinetics regarding two herbal feed plant additives. The tested herbal mixtures were elaborated with Phaseolus mango and Linum usitatissimum, providing lysine (Lys) and Trigonella foenum-graecum and Allium sativa, providing Methionine (Met). They were compared to alfalfa (Medicago sativa) and solvent extracted soybean meal (Glicine max), as standard sources of protein using the in vitro gas production technique modified to estimate N-NH3, recording fermentation kinetics and dry matter digestibility (72 h), in a completely randomized design followed by Tukey test. Ruminal ammonia concentration in the herbal mixtures was lower (P<0.05) than in the standard protein sources, indicating that protein from herbal mixtures could resist ruminal degradation. Herbal additives with Lys or Met showed minimum N-NH3 concentration in the first 4 h of incubation. At 8 h, the concentration was 0.27 and 0.54 mg dL-1 for the herbal products with Lys and Met, significantly lower than solvent extracted soybean meal and alfalfa (1.15 and 2.24 mg dL-1 respectively, P<0.05). Highlights The tested herbal mixture elaborated with Phaseolus mango and Linum usitatissimum, provide bypass Lysine. The tested herbal mixture elaborated with Trigonella foenum-graecum and Allium sativa, provide bypass Methionine. Ruminal ammonia concentration in the herbal mixtures was lower than in the standard protein sources. Protein from herbal mixtures could resist ruminal degradation.