SUPLEMENTASI FETAL BOVINE SERUM (FBS) TERHADAP PERTUMBUHAN IN VITRO SEL FOLIKEL KAMBING PE

• Main Authors: S.N Rahaju, S. Wahjuningsih
• Format: Article
• Language: Indonesian
• Published: University of Brawijaya 2012-04-01
• Series: Jurnal Ternak Tropika
• Online Access: http://ternaktropika.ub.ac.id/index.php/tropika/article/view/137

Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan suplementasi FBS dengan berbagai konsentrasi pada medium kultur Tissue Culture Medium-199 (TCM-199) dengan variasi waktu inkubasi terhadap kompetensi pertumbuhan folikel kambing PE. Materi Penelitian adalah folikel kambing yang diperoleh melalui isolasi ovarium kambing, TCM-199 dan FBS. Analisa data menggunakan uji t berpasangan dengan menggunakan 3 perlakuan suplementasi FBS (0%, 0,1% dan 1%) dan inkubasi selama 6 hari. Folikel yang digunakan dalam kultur dikelompokkan menjadi 2 ukuran yaitu besar (4mm) dan kecil (2-<4mm). Variabel pengamatan yang digunakan adalah pertumbuhan folikel. Pengamatan dilakukan pada hari ke 0 dan hari ke 6. Hasil penelitian menunjukkan bahwa suplementasi FBS pada kultur folikel secara IVG memberikan pengaruh nyata (P<0,05) terhadap pertumbuhan folikel, yaitu pada folikel besar dengan suplementasi FBS 1% dan folikel kecil dengan suplementasi FBS 0,1%. Kata Kunci : Fetal Bovine Serum, Folikel, Pertumbuhan In Vitro, Kambing FETAL BOVINE SERUM (FBS) SUPPLEMENTATION WITH INCUBATION TIME VARIATION TO FOLLICLE GROWTH COMPETENCE OF PERANAKAN ETAWAH (PE) GOAT ABSTRACT The aims of this research was to know the effect of FBS supplements usage with any concentration of Tissue Culture Medium-199 (TCM-199) culture medium with incubation time variation to follicle growth competence of Peranakan Etawah (PE) goat. The material of this research were goat follicle, TCM-199 and FBS. The method of this research use t test which used 3 treatment with incubation 6 days. The follicle that used in culture were classified into 2 groups: large (>4mm) and small (2- <4mm). The observation variable were used follicle growth. The result of this research are showed that FBS supplements in follicle culture according to IVG gave the significant different into follicle growth (P<0,05) that is large follicle with 1% FBS supplements and small follicle with 0,1% FBS supplements. It can be concluded that FBS supplements and length of culture in IVG follicle had the significant influence on the growth of follicles. Keywords: Fetal Bovine Serum,Follicle, In Vitro Growth,Goat