Polymorphism of FcyRIIIA-l58F/V and promotion region of IL-10 genes in systemic lupus erythematosus in Kazakhs

• Main Authors: I A Guseva, Zh E Omarbekova, V A Myakotkin
• Format: Article
• Language: Russian
• Published: "Consilium Medicum" Publishing house 2003-05-01
• Series: Терапевтический архив
• Subjects: il-10; fcy-receptor; genetic polymorphism; systemic lupus erythematosus
• Online Access: https://ter-arkhiv.ru/0040-3660/article/view/29367
• ISSN: 0040-3660; 2309-5342

Aim. To assess the role of allele polymorphism of genes FcyRlH and IL-10 in systemic lupus erythematosus (SLR) in a homogeneous (stratified by nationality) Kazakh population sample. Material and methods. Polymorphism of genes FcyRIHA and IL-10 was studied in 49 patients SLE patients and 81 healthy subjects (control group). Detection of two allele variants of gene FcyRIHA (alleles F and V) caused by point mutation in position 559 was performed by polymerase chain reaction (PCR) in the amplification refractory mutation system. Genetic changeability of IL-10 gene promotion site due to point mutation in position 627 (alleles I and 2) was investigated by polymorphism of restricted fragment lengths using restrictase Rsal. Results. Significant differences in the distribution rates of genes FcyRIHA and IL-10 were revealed in SLE patients versus controls. Alleles F and -627A(l), including homozygous ones, are genes predisposing to development of SLE while variants of the markers V and -627(2) are protecting genes. If a SLE patient's genotype combines alleles F of gene FcyRIII and alleles 1 of gene IL-10 (F/F+l/1), this patient has an 8 times higher risk to develop SLE. Conclusion. Polymorphism of genes FcyRIHA and IL-10 is associated with predisposition to development of SLE in Kazakh population. The analysis of combined genotypes of the studied genes suggests a synergic action of genes FcyRIHA and IL-10 on the risk to develop SLE.