Effect of collection, transport, processing and storage of blood specimens on the activity of lysosomal enzymes in plasma and leukocytes

• Main Authors: M. Burin, C. Dutra-Filho, J. Brum, T. Mauricio, M. Amorim, R. Giugliani
• Format: Article
• Language: English
• Published: Associação Brasileira de Divulgação Científica 2000-09-01
• Series: Brazilian Journal of Medical and Biological Research
• Subjects: lysosomal storage diseases; lysosomal enzymes; inborn errors of metabolism; reference laboratories; storage of blood samples; sample handling
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900003
• ISSN: 0100-879X; 1414-431X

This study was designed to evaluate the effect of different conditions of collection, transport and storage on the quality of blood samples from normal individuals in terms of the activity of the enzymes ß-glucuronidase, total hexosaminidase, hexosaminidase A, arylsulfatase A and ß-galactosidase. The enzyme activities were not affected by the different materials used for collection (plastic syringes or vacuum glass tubes). In the evaluation of different heparin concentrations (10% heparin, 5% heparin, and heparinized syringe) in the syringes, it was observed that higher doses resulted in an increase of at least 1-fold in the activities of ß-galactosidase, total hexosaminidase and hexosaminidase A in leukocytes, and ß-glucuronidase in plasma. When the effects of time and means of transportation were studied, samples that had been kept at room temperature showed higher deterioration with time (72 and 96 h) before processing, and in this case it was impossible to isolate leukocytes from most samples. Comparison of heparin and acid citrate-dextrose (ACD) as anticoagulants revealed that ß-glucuronidase and hexosaminidase activities in plasma reached levels near the lower normal limits when ACD was used. In conclusion, we observed that heparin should be used as the preferable anticoagulant when measuring these lysosomal enzyme activities, and we recommend that, when transport time is more than 24 h, samples should be shipped by air in a styrofoam box containing wet ice.