An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

As the COVID-19 pandemic continues, variants of the virus are emerging. Here the authors present a diagnostic assay that can detect wildtype and known variants using engineered Cas12a.

Bibliographic Details
Main Authors: Kean Hean Ooi, Mengying Mandy Liu, Jie Wen Douglas Tay, Seok Yee Teo, Pornchai Kaewsapsak, Shengyang Jin, Chun Kiat Lee, Jingwen Hou, Sebastian Maurer-Stroh, Weisi Lin, Benedict Yan, Gabriel Yan, Yong-Gui Gao, Meng How Tan
Format: Article
Language:English
Published: Nature Publishing Group 2021-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-21996-6
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spelling doaj-caf7de7ec01a4742a1eed676b48f380b2021-03-21T12:14:31ZengNature Publishing GroupNature Communications2041-17232021-03-0112112310.1038/s41467-021-21996-6An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testingKean Hean Ooi0Mengying Mandy Liu1Jie Wen Douglas Tay2Seok Yee Teo3Pornchai Kaewsapsak4Shengyang Jin5Chun Kiat Lee6Jingwen Hou7Sebastian Maurer-Stroh8Weisi Lin9Benedict Yan10Gabriel Yan11Yong-Gui Gao12Meng How Tan13School of Chemical and Biomedical Engineering, Nanyang Technological UniversitySchool of Chemical and Biomedical Engineering, Nanyang Technological UniversitySchool of Chemical and Biomedical Engineering, Nanyang Technological UniversitySchool of Chemical and Biomedical Engineering, Nanyang Technological UniversityGenome Institute of Singapore, Agency for Science Technology and ResearchSchool of Biological Sciences, Nanyang Technological UniversityDepartment of Laboratory Medicine, National University Hospital, National University Health SystemSchool of Computer Science and Engineering, Nanyang Technological UniversityBioinformatics Institute, Agency for Science Technology and ResearchSchool of Computer Science and Engineering, Nanyang Technological UniversityDepartment of Laboratory Medicine, National University Hospital, National University Health SystemDivision of Infectious Diseases, Department of Medicine, National University Hospital, National University Health SystemSchool of Biological Sciences, Nanyang Technological UniversitySchool of Chemical and Biomedical Engineering, Nanyang Technological UniversityAs the COVID-19 pandemic continues, variants of the virus are emerging. Here the authors present a diagnostic assay that can detect wildtype and known variants using engineered Cas12a.https://doi.org/10.1038/s41467-021-21996-6
collection DOAJ
language English
format Article
sources DOAJ
author Kean Hean Ooi
Mengying Mandy Liu
Jie Wen Douglas Tay
Seok Yee Teo
Pornchai Kaewsapsak
Shengyang Jin
Chun Kiat Lee
Jingwen Hou
Sebastian Maurer-Stroh
Weisi Lin
Benedict Yan
Gabriel Yan
Yong-Gui Gao
Meng How Tan
spellingShingle Kean Hean Ooi
Mengying Mandy Liu
Jie Wen Douglas Tay
Seok Yee Teo
Pornchai Kaewsapsak
Shengyang Jin
Chun Kiat Lee
Jingwen Hou
Sebastian Maurer-Stroh
Weisi Lin
Benedict Yan
Gabriel Yan
Yong-Gui Gao
Meng How Tan
An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
Nature Communications
author_facet Kean Hean Ooi
Mengying Mandy Liu
Jie Wen Douglas Tay
Seok Yee Teo
Pornchai Kaewsapsak
Shengyang Jin
Chun Kiat Lee
Jingwen Hou
Sebastian Maurer-Stroh
Weisi Lin
Benedict Yan
Gabriel Yan
Yong-Gui Gao
Meng How Tan
author_sort Kean Hean Ooi
title An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
title_short An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
title_full An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
title_fullStr An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
title_full_unstemmed An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
title_sort engineered crispr-cas12a variant and dna-rna hybrid guides enable robust and rapid covid-19 testing
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-03-01
description As the COVID-19 pandemic continues, variants of the virus are emerging. Here the authors present a diagnostic assay that can detect wildtype and known variants using engineered Cas12a.
url https://doi.org/10.1038/s41467-021-21996-6
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