Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury
We have designed a novel nerve guidance conduit (NGC) made from silk fibroin and poly(lactic-co-glycolic acid) through electrospinning and weaving (ESP-NGCs). Several physical and biological properties of the ESP-NGCs were assessed in order to evaluate their biocompatibility. The physical properties...
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Wolters Kluwer Medknow Publications
2015-01-01
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doaj-ca8e59b0d4d44d8abe9f7cbd7ae7c7902020-11-25T03:20:35ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742015-01-0110101635164210.4103/1673-5374.167763Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injuryYa-ling WangXiao-mei GuYan KongQi-lin FengYu-min YangWe have designed a novel nerve guidance conduit (NGC) made from silk fibroin and poly(lactic-co-glycolic acid) through electrospinning and weaving (ESP-NGCs). Several physical and biological properties of the ESP-NGCs were assessed in order to evaluate their biocompatibility. The physical properties, including thickness, tensile stiffness, infrared spectroscopy, porosity, and water absorption were determined in vitro. To assess the biological properties, Schwann cells were cultured in ESP-NGC extracts and were assessed by morphological observation, the MTT assay, and immunohistochemistry. In addition, ESP-NGCs were subcutaneously implanted in the backs of rabbits to evaluate their biocompatibility in vivo. The results showed that ESP-NGCs have high porosity, strong hydrophilicity, and strong tensile stiffness. Schwann cells cultured in the ESP-NGC extract fluids showed no significant differences compared to control cells in their morphology or viability. Histological evaluation of the ESP-NGCs implanted in vivo indicated a mild inflammatory reaction and high biocompatibility. Together, these data suggest that these novel ESP-NGCs are biocompatible, and may thus provide a reliable scaffold for peripheral nerve repair in clinical application.http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=10;spage=1635;epage=1642;aulast=Wangnerve regeneration; peripheral nerve injury; poly(lactic-co-glycolic acid); electrospinning; silk fibroin; biocompatibility; nerve guidance conduit; weaving |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ya-ling Wang Xiao-mei Gu Yan Kong Qi-lin Feng Yu-min Yang |
spellingShingle |
Ya-ling Wang Xiao-mei Gu Yan Kong Qi-lin Feng Yu-min Yang Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury Neural Regeneration Research nerve regeneration; peripheral nerve injury; poly(lactic-co-glycolic acid); electrospinning; silk fibroin; biocompatibility; nerve guidance conduit; weaving |
author_facet |
Ya-ling Wang Xiao-mei Gu Yan Kong Qi-lin Feng Yu-min Yang |
author_sort |
Ya-ling Wang |
title |
Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury |
title_short |
Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury |
title_full |
Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury |
title_fullStr |
Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury |
title_full_unstemmed |
Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury |
title_sort |
electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury |
publisher |
Wolters Kluwer Medknow Publications |
series |
Neural Regeneration Research |
issn |
1673-5374 |
publishDate |
2015-01-01 |
description |
We have designed a novel nerve guidance conduit (NGC) made from silk fibroin and poly(lactic-co-glycolic acid) through electrospinning and weaving (ESP-NGCs). Several physical and biological properties of the ESP-NGCs were assessed in order to evaluate their biocompatibility. The physical properties, including thickness, tensile stiffness, infrared spectroscopy, porosity, and water absorption were determined in vitro. To assess the biological properties, Schwann cells were cultured in ESP-NGC extracts and were assessed by morphological observation, the MTT assay, and immunohistochemistry. In addition, ESP-NGCs were subcutaneously implanted in the backs of rabbits to evaluate their biocompatibility in vivo. The results showed that ESP-NGCs have high porosity, strong hydrophilicity, and strong tensile stiffness. Schwann cells cultured in the ESP-NGC extract fluids showed no significant differences compared to control cells in their morphology or viability. Histological evaluation of the ESP-NGCs implanted in vivo indicated a mild inflammatory reaction and high biocompatibility. Together, these data suggest that these novel ESP-NGCs are biocompatible, and may thus provide a reliable scaffold for peripheral nerve repair in clinical application. |
topic |
nerve regeneration; peripheral nerve injury; poly(lactic-co-glycolic acid); electrospinning; silk fibroin; biocompatibility; nerve guidance conduit; weaving |
url |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=10;spage=1635;epage=1642;aulast=Wang |
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