Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury

We have designed a novel nerve guidance conduit (NGC) made from silk fibroin and poly(lactic-co-glycolic acid) through electrospinning and weaving (ESP-NGCs). Several physical and biological properties of the ESP-NGCs were assessed in order to evaluate their biocompatibility. The physical properties...

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Main Authors: Ya-ling Wang, Xiao-mei Gu, Yan Kong, Qi-lin Feng, Yu-min Yang
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2015-01-01
Series:Neural Regeneration Research
Subjects:
Online Access:http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=10;spage=1635;epage=1642;aulast=Wang
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spelling doaj-ca8e59b0d4d44d8abe9f7cbd7ae7c7902020-11-25T03:20:35ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742015-01-0110101635164210.4103/1673-5374.167763Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injuryYa-ling WangXiao-mei GuYan KongQi-lin FengYu-min YangWe have designed a novel nerve guidance conduit (NGC) made from silk fibroin and poly(lactic-co-glycolic acid) through electrospinning and weaving (ESP-NGCs). Several physical and biological properties of the ESP-NGCs were assessed in order to evaluate their biocompatibility. The physical properties, including thickness, tensile stiffness, infrared spectroscopy, porosity, and water absorption were determined in vitro. To assess the biological properties, Schwann cells were cultured in ESP-NGC extracts and were assessed by morphological observation, the MTT assay, and immunohistochemistry. In addition, ESP-NGCs were subcutaneously implanted in the backs of rabbits to evaluate their biocompatibility in vivo. The results showed that ESP-NGCs have high porosity, strong hydrophilicity, and strong tensile stiffness. Schwann cells cultured in the ESP-NGC extract fluids showed no significant differences compared to control cells in their morphology or viability. Histological evaluation of the ESP-NGCs implanted in vivo indicated a mild inflammatory reaction and high biocompatibility. Together, these data suggest that these novel ESP-NGCs are biocompatible, and may thus provide a reliable scaffold for peripheral nerve repair in clinical application.http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=10;spage=1635;epage=1642;aulast=Wangnerve regeneration; peripheral nerve injury; poly(lactic-co-glycolic acid); electrospinning; silk fibroin; biocompatibility; nerve guidance conduit; weaving
collection DOAJ
language English
format Article
sources DOAJ
author Ya-ling Wang
Xiao-mei Gu
Yan Kong
Qi-lin Feng
Yu-min Yang
spellingShingle Ya-ling Wang
Xiao-mei Gu
Yan Kong
Qi-lin Feng
Yu-min Yang
Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury
Neural Regeneration Research
nerve regeneration; peripheral nerve injury; poly(lactic-co-glycolic acid); electrospinning; silk fibroin; biocompatibility; nerve guidance conduit; weaving
author_facet Ya-ling Wang
Xiao-mei Gu
Yan Kong
Qi-lin Feng
Yu-min Yang
author_sort Ya-ling Wang
title Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury
title_short Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury
title_full Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury
title_fullStr Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury
title_full_unstemmed Electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury
title_sort electrospun and woven silk fibroin/poly(lactic-co- glycolic acid) nerve guidance conduits for repairing peripheral nerve injury
publisher Wolters Kluwer Medknow Publications
series Neural Regeneration Research
issn 1673-5374
publishDate 2015-01-01
description We have designed a novel nerve guidance conduit (NGC) made from silk fibroin and poly(lactic-co-glycolic acid) through electrospinning and weaving (ESP-NGCs). Several physical and biological properties of the ESP-NGCs were assessed in order to evaluate their biocompatibility. The physical properties, including thickness, tensile stiffness, infrared spectroscopy, porosity, and water absorption were determined in vitro. To assess the biological properties, Schwann cells were cultured in ESP-NGC extracts and were assessed by morphological observation, the MTT assay, and immunohistochemistry. In addition, ESP-NGCs were subcutaneously implanted in the backs of rabbits to evaluate their biocompatibility in vivo. The results showed that ESP-NGCs have high porosity, strong hydrophilicity, and strong tensile stiffness. Schwann cells cultured in the ESP-NGC extract fluids showed no significant differences compared to control cells in their morphology or viability. Histological evaluation of the ESP-NGCs implanted in vivo indicated a mild inflammatory reaction and high biocompatibility. Together, these data suggest that these novel ESP-NGCs are biocompatible, and may thus provide a reliable scaffold for peripheral nerve repair in clinical application.
topic nerve regeneration; peripheral nerve injury; poly(lactic-co-glycolic acid); electrospinning; silk fibroin; biocompatibility; nerve guidance conduit; weaving
url http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=10;spage=1635;epage=1642;aulast=Wang
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