SDF1-induced antagonism of axonal repulsion requires multiple G-protein coupled signaling components that work in parallel.

SDF1-induced antagonism of axonal repulsion requires multiple G-protein coupled signaling components that work in parallel.

SDF1 reduces the responsiveness of axonal growth cones to repellent guidance cues in a pertussis-toxin-sensitive, cAMP-dependent manner. Here, we show that SDF1's antirepellent effect can be blocked in embryonic chick dorsal root ganglia (DRGs) by expression of peptides or proteins inhibiting e...

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Main Authors: E Naomi Twery, Jonathan A Raper
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-04-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3083402?pdf=render
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spelling doaj-ca7f65fd54894b18955915805ac6b3982020-11-25T01:52:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-04-0164e1889610.1371/journal.pone.0018896SDF1-induced antagonism of axonal repulsion requires multiple G-protein coupled signaling components that work in parallel.E Naomi TweryJonathan A RaperSDF1 reduces the responsiveness of axonal growth cones to repellent guidance cues in a pertussis-toxin-sensitive, cAMP-dependent manner. Here, we show that SDF1's antirepellent effect can be blocked in embryonic chick dorsal root ganglia (DRGs) by expression of peptides or proteins inhibiting either Gα(i), Gα(q), or Gβγ. SDF1 antirepellent activity is also blocked by pharmacological inhibition of PLC, a common effector protein for Gα(q). We also show that SDF1 antirepellent activity can be mimicked by overexpression of constitutively active Gα(i), Gα(q), or Gα(s). These results suggest a model in which multiple G protein components cooperate to produce the cAMP levels required for SDF1 antirepellent activity.http://europepmc.org/articles/PMC3083402?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author E Naomi Twery
Jonathan A Raper
spellingShingle E Naomi Twery
Jonathan A Raper
SDF1-induced antagonism of axonal repulsion requires multiple G-protein coupled signaling components that work in parallel.
PLoS ONE
author_facet E Naomi Twery
Jonathan A Raper
author_sort E Naomi Twery
title SDF1-induced antagonism of axonal repulsion requires multiple G-protein coupled signaling components that work in parallel.
title_short SDF1-induced antagonism of axonal repulsion requires multiple G-protein coupled signaling components that work in parallel.
title_full SDF1-induced antagonism of axonal repulsion requires multiple G-protein coupled signaling components that work in parallel.
title_fullStr SDF1-induced antagonism of axonal repulsion requires multiple G-protein coupled signaling components that work in parallel.
title_full_unstemmed SDF1-induced antagonism of axonal repulsion requires multiple G-protein coupled signaling components that work in parallel.
title_sort sdf1-induced antagonism of axonal repulsion requires multiple g-protein coupled signaling components that work in parallel.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2011-04-01
description SDF1 reduces the responsiveness of axonal growth cones to repellent guidance cues in a pertussis-toxin-sensitive, cAMP-dependent manner. Here, we show that SDF1's antirepellent effect can be blocked in embryonic chick dorsal root ganglia (DRGs) by expression of peptides or proteins inhibiting either Gα(i), Gα(q), or Gβγ. SDF1 antirepellent activity is also blocked by pharmacological inhibition of PLC, a common effector protein for Gα(q). We also show that SDF1 antirepellent activity can be mimicked by overexpression of constitutively active Gα(i), Gα(q), or Gα(s). These results suggest a model in which multiple G protein components cooperate to produce the cAMP levels required for SDF1 antirepellent activity.
url http://europepmc.org/articles/PMC3083402?pdf=render
work_keys_str_mv AT enaomitwery sdf1inducedantagonismofaxonalrepulsionrequiresmultiplegproteincoupledsignalingcomponentsthatworkinparallel
AT jonathanaraper sdf1inducedantagonismofaxonalrepulsionrequiresmultiplegproteincoupledsignalingcomponentsthatworkinparallel
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