Antioxidant activity of mandarin (Citrus reticulata) peel

• Main Authors: Tumbas Vesna T., Ćetković Gordana S., Đilas Sonja M., Čanadanović-Brunet Jasna M., Vulić Jelena J., Knez Željko, Škerget Mojca
• Format: Article
• Language: English
• Published: Faculty of Technology, Novi Sad 2010-01-01
• Series: Acta Periodica Technologica
• Subjects: Mandarin peel; DPPH radical; hydroxyl radical; TBARS
• Online Access: http://www.doiserbia.nb.rs/img/doi/1450-7188/2010/1450-71881041195T.pdf

Mandarin peel, a waste product coming from juice production, was extracted by conventional extraction with 70% acetone. Content of flavonoids in mandarin peel extract (MPE) was determined by HPLC. Hesperidin was the most dominant flavonoid. Free radical scavenging activity of MPE on stable DPPH radicals and reactive hydroxyl radicals was also evaluated. EC50 value determined in spectrophotometrical DPPH radical assay was 0.179 mg/ml, while this value in ESR spin trapping hydroxyl radical assay was 0.415 mg/ml. Also, MPE showed protective effects in stabilising sunflower oil during accelerated storage. The results indicated that mandarin peel can be a valuable source of natural antioxidants.