Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin

Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin

Abstract Octenidine dihydrochloride (OCT) is a widely used antiseptic molecule, promoting skin wound healing accompanied with improved scar quality after surgical procedures. However, the mechanisms by which OCT is contributing to tissue regeneration are not yet completely clear. In this study, we h...

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Main Authors: Saskia Seiser, Lukas Janker, Nina Zila, Michael Mildner, Ana Rakita, Johannes Matiasek, Andrea Bileck, Christopher Gerner, Verena Paulitschke, Adelheid Elbe-Bürger
Format: Article
Language:English
Published: Nature Publishing Group 2021-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-79378-9
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spelling doaj-ca6e0d3da544492aad3a726a3e29cf092021-01-10T12:48:44ZengNature Publishing GroupScientific Reports2045-23222021-01-0111111310.1038/s41598-020-79378-9Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skinSaskia Seiser0Lukas Janker1Nina Zila2Michael Mildner3Ana Rakita4Johannes Matiasek5Andrea Bileck6Christopher Gerner7Verena Paulitschke8Adelheid Elbe-Bürger9Department of Dermatology, Medical University of ViennaDepartment of Analytical Chemistry, University of ViennaDepartment of Dermatology, Medical University of ViennaDepartment of Dermatology, Medical University of ViennaDepartment of Dermatology, Medical University of ViennaDepartment of Plastic, Aesthetic and Reconstructive Surgery, St. Josef HospitalDepartment of Analytical Chemistry, University of ViennaDepartment of Analytical Chemistry, University of ViennaDepartment of Dermatology, Medical University of ViennaDepartment of Dermatology, Medical University of ViennaAbstract Octenidine dihydrochloride (OCT) is a widely used antiseptic molecule, promoting skin wound healing accompanied with improved scar quality after surgical procedures. However, the mechanisms by which OCT is contributing to tissue regeneration are not yet completely clear. In this study, we have used a superficial wound model by tape stripping of ex vivo human skin. Protein profiles of wounded skin biopsies treated with OCT-containing hydrogel and the released secretome were analyzed using liquid chromatography-mass spectrometry (LC–MS) and enzyme-linked immunosorbent assay (ELISA), respectively. Proteomics analysis of OCT-treated skin wounds revealed significant lower levels of key players in tissue remodeling as well as reepithelization after wounding such as pro-inflammatory cytokines (IL-8, IL-6) and matrix-metalloproteinases (MMP1, MMP2, MMP3, MMP9) when compared to controls. In addition, enzymatic activity of several released MMPs into culture supernatants was significantly lower in OCT-treated samples. Our data give insights on the mode of action based on which OCT positively influences wound healing and identified anti-inflammatory and protease-inhibitory activities of OCT.https://doi.org/10.1038/s41598-020-79378-9
collection DOAJ
language English
format Article
sources DOAJ
author Saskia Seiser
Lukas Janker
Nina Zila
Michael Mildner
Ana Rakita
Johannes Matiasek
Andrea Bileck
Christopher Gerner
Verena Paulitschke
Adelheid Elbe-Bürger
spellingShingle Saskia Seiser
Lukas Janker
Nina Zila
Michael Mildner
Ana Rakita
Johannes Matiasek
Andrea Bileck
Christopher Gerner
Verena Paulitschke
Adelheid Elbe-Bürger
Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
Scientific Reports
author_facet Saskia Seiser
Lukas Janker
Nina Zila
Michael Mildner
Ana Rakita
Johannes Matiasek
Andrea Bileck
Christopher Gerner
Verena Paulitschke
Adelheid Elbe-Bürger
author_sort Saskia Seiser
title Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
title_short Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
title_full Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
title_fullStr Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
title_full_unstemmed Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
title_sort octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-01-01
description Abstract Octenidine dihydrochloride (OCT) is a widely used antiseptic molecule, promoting skin wound healing accompanied with improved scar quality after surgical procedures. However, the mechanisms by which OCT is contributing to tissue regeneration are not yet completely clear. In this study, we have used a superficial wound model by tape stripping of ex vivo human skin. Protein profiles of wounded skin biopsies treated with OCT-containing hydrogel and the released secretome were analyzed using liquid chromatography-mass spectrometry (LC–MS) and enzyme-linked immunosorbent assay (ELISA), respectively. Proteomics analysis of OCT-treated skin wounds revealed significant lower levels of key players in tissue remodeling as well as reepithelization after wounding such as pro-inflammatory cytokines (IL-8, IL-6) and matrix-metalloproteinases (MMP1, MMP2, MMP3, MMP9) when compared to controls. In addition, enzymatic activity of several released MMPs into culture supernatants was significantly lower in OCT-treated samples. Our data give insights on the mode of action based on which OCT positively influences wound healing and identified anti-inflammatory and protease-inhibitory activities of OCT.
url https://doi.org/10.1038/s41598-020-79378-9
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