High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland

High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland

Mastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples fro...

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Main Authors: Bozena Dworecka-Kaszak, Alicja Krutkiewicz, Daniel Szopa, Miroslaw Kleczkowski, Malgorzata Biegańska
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/196347
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spelling doaj-ca59b24c929c4303a24c85248781658c2020-11-24T21:48:59ZengHindawi LimitedThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/196347196347High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in PolandBozena Dworecka-Kaszak0Alicja Krutkiewicz1Daniel Szopa2Miroslaw Kleczkowski3Malgorzata Biegańska4Mycology Division, Preclinical Science Department, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-789 Warsaw, PolandMycology Division, Preclinical Science Department, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-789 Warsaw, PolandMolecular Genetic Division, Mother and Child institute, Kasprzaka 17a, 01-211 Warsaw, PolandDivision of Laboratory Diagnostics, Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, PolandMycology Division, Preclinical Science Department, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-789 Warsaw, PolandMastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples from mastitic cows were studied, and 55 strains of fungi were isolated. Seven different species classified as Candida were identified basing on phenotypic properties, and the dominating species was C. parapsilosis. Genomic DNA was isolated and amplified in PCR with ITS1 and NL2 primers. Amplification products were digested with restriction enzymes HpaII and EcoRI. Amplification of DNA with ITS1 and NL2 primers resulted in products of different sizes. Comparison of product sizes in restriction fragment PCR REA confirmed differences among species. Strains grouped together on the basis of phenotype characteristics differed in restriction fragment profiles. None of the investigated species showed similar genetic profiles.http://dx.doi.org/10.1100/2012/196347
collection DOAJ
language English
format Article
sources DOAJ
author Bozena Dworecka-Kaszak
Alicja Krutkiewicz
Daniel Szopa
Miroslaw Kleczkowski
Malgorzata Biegańska
spellingShingle Bozena Dworecka-Kaszak
Alicja Krutkiewicz
Daniel Szopa
Miroslaw Kleczkowski
Malgorzata Biegańska
High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
The Scientific World Journal
author_facet Bozena Dworecka-Kaszak
Alicja Krutkiewicz
Daniel Szopa
Miroslaw Kleczkowski
Malgorzata Biegańska
author_sort Bozena Dworecka-Kaszak
title High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
title_short High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
title_full High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
title_fullStr High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
title_full_unstemmed High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland
title_sort high prevalence of candida yeast in milk samples from cows suffering from mastitis in poland
publisher Hindawi Limited
series The Scientific World Journal
issn 1537-744X
publishDate 2012-01-01
description Mastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples from mastitic cows were studied, and 55 strains of fungi were isolated. Seven different species classified as Candida were identified basing on phenotypic properties, and the dominating species was C. parapsilosis. Genomic DNA was isolated and amplified in PCR with ITS1 and NL2 primers. Amplification products were digested with restriction enzymes HpaII and EcoRI. Amplification of DNA with ITS1 and NL2 primers resulted in products of different sizes. Comparison of product sizes in restriction fragment PCR REA confirmed differences among species. Strains grouped together on the basis of phenotype characteristics differed in restriction fragment profiles. None of the investigated species showed similar genetic profiles.
url http://dx.doi.org/10.1100/2012/196347
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AT miroslawkleczkowski highprevalenceofcandidayeastinmilksamplesfromcowssufferingfrommastitisinpoland
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