Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry

The aim of the study is to better understand where uranium deposits in mice kidneys. The spatial distribution of uranium was examined in the kidneys of C57BL/6 mice using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Mice were exposed to varying levels of uranyl nitrate in...

Full description

Bibliographic Details
Main Authors:	Venessa Jim, Corinne LaViolette, Margaret M. Briehl, Jani C. Ingram
Format:	Article
Language:	English
Published:	Betasciencepress Publishers 2017-06-01
Series:	Journal of Applied Bioanalysis
Subjects:	uranium microprobe mice kidney LA-ICP-MS
Online Access:	https://doi.org/10.17145/jab.17.007

id	doaj-ca2de687f9df48f3baeb844d46ec9aa9
record_format	Article
spelling	doaj-ca2de687f9df48f3baeb844d46ec9aa92020-11-25T02:19:16ZengBetasciencepress PublishersJournal of Applied Bioanalysis2405-710X2017-06-0133434810.17145/jab.17.007Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometryVenessa Jim0Corinne LaViolette1Margaret M. Briehl2Jani C. Ingram3Department of Chemistry & Biochemistry, Northern Arizona University, Flagstaff, USADepartment of Chemistry & Biochemistry, Northern Arizona University, Flagstaff, USADepartment of Pathology, University of Arizona, Tucson, USADepartment of Chemistry & Biochemistry, Northern Arizona University, Flagstaff, USAThe aim of the study is to better understand where uranium deposits in mice kidneys. The spatial distribution of uranium was examined in the kidneys of C57BL/6 mice using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Mice were exposed to varying levels of uranyl nitrate in their drinking water. Calibration standards were developed to allow for semi-quantitative measurement of uranium in the cortical and medullary regions of mice kidney by LA-ICP-MS. Scanning electron microscopy was used to image the ablation patterns on the kidney. Uranium levels were observed to increase in kidney tissue as uranyl nitrate treatment exposure levels increased. A trend towards a higher uranium concentration in the medullary versus cortical region of the kidneys was observed. These results show the usefulness of LA-ICP-MS in toxicity studies by providing a quantitative, spatial assessment of uranium deposition in a target organ.https://doi.org/10.17145/jab.17.007uraniummicroprobemice kidneyLA-ICP-MS
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Venessa Jim Corinne LaViolette Margaret M. Briehl Jani C. Ingram
spellingShingle	Venessa Jim Corinne LaViolette Margaret M. Briehl Jani C. Ingram Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry Journal of Applied Bioanalysis uranium microprobe mice kidney LA-ICP-MS
author_facet	Venessa Jim Corinne LaViolette Margaret M. Briehl Jani C. Ingram
author_sort	Venessa Jim
title	Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry
title_short	Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry
title_full	Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry
title_fullStr	Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry
title_full_unstemmed	Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry
title_sort	spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry
publisher	Betasciencepress Publishers
series	Journal of Applied Bioanalysis
issn	2405-710X
publishDate	2017-06-01
description	The aim of the study is to better understand where uranium deposits in mice kidneys. The spatial distribution of uranium was examined in the kidneys of C57BL/6 mice using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Mice were exposed to varying levels of uranyl nitrate in their drinking water. Calibration standards were developed to allow for semi-quantitative measurement of uranium in the cortical and medullary regions of mice kidney by LA-ICP-MS. Scanning electron microscopy was used to image the ablation patterns on the kidney. Uranium levels were observed to increase in kidney tissue as uranyl nitrate treatment exposure levels increased. A trend towards a higher uranium concentration in the medullary versus cortical region of the kidneys was observed. These results show the usefulness of LA-ICP-MS in toxicity studies by providing a quantitative, spatial assessment of uranium deposition in a target organ.
topic	uranium microprobe mice kidney LA-ICP-MS
url	https://doi.org/10.17145/jab.17.007
work_keys_str_mv	AT venessajim spatialdistributionofuraniuminmicekidneysdetectedbylaserablationinductivelycoupledplasmamassspectrometry AT corinnelaviolette spatialdistributionofuraniuminmicekidneysdetectedbylaserablationinductivelycoupledplasmamassspectrometry AT margaretmbriehl spatialdistributionofuraniuminmicekidneysdetectedbylaserablationinductivelycoupledplasmamassspectrometry AT janicingram spatialdistributionofuraniuminmicekidneysdetectedbylaserablationinductivelycoupledplasmamassspectrometry
_version_	1724877131353161728

Spatial distribution of uranium in mice kidneys detected by laser ablation inductively coupled plasma mass spectrometry

Similar Items