Composition of the Oral Microbiome in Patients with Coronary Artery Disease
Introduction: Distinct microbial communities reside in the oral cavity and the composition of the oral microbiota has important implications for human health and disease. Identification of bacterial flora of the microbiome is done by metagenomic analysis of 16S ribosomal RNA sequences. Aim: The...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2020-09-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/14034/45246_CE[Ra1]_F(SHU)_PF1(AG_KM)_PFA(KM)_PB(AG_KM)_PN(SL).pdf |
| Summary: | Introduction: Distinct microbial communities reside in the oral
cavity and the composition of the oral microbiota has important
implications for human health and disease. Identification of
bacterial flora of the microbiome is done by metagenomic
analysis of 16S ribosomal RNA sequences.
Aim: The aim of this study was to characterise the human
microbiome in patients with Coronary Artery Disease (CAD) in
comparison with the normal human microbiome.
Materials and Methods: A pilot study was carried out in tertiary
hospital, Chennai. Oral mouthwash samples collected from
nine patients with CAD were selected, with one control group.
They were studied by metagenomic analysis of V3-V4 region of
16SrRNA gene sequences.. Sequencing of the variable V3 and
V4 regions was done using Illumina platform.
Results: The six major phyla, Firmicutes, Bacteroidetes,
Proteobacteria, Actinobacteria, Spirochaetes, and Fusobacteria
contained 99% of the taxa in all the samples analysed.
Conclusion: Diversity of the microbiome in patients with CAD
was similar to the normal human microbiome. |
|---|---|
| ISSN: | 2249-782X 0973-709X |