Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy

Light-sheet fluorescence microscopy enables high resolution imaging of biological samples. Here the authors use reflective coverslips to obtain multiple sample views simultaneously, improving the speed of acquisition and resolution compared to dual-view selective plane illumination microscopy.

Bibliographic Details
Main Authors:	Yicong Wu, Abhishek Kumar, Corey Smith, Evan Ardiel, Panagiotis Chandris, Ryan Christensen, Ivan Rey-Suarez, Min Guo, Harshad D. Vishwasrao, Jiji Chen, Jianyong Tang, Arpita Upadhyaya, Patrick J. La Riviere, Hari Shroff
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-11-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-017-01250-8

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spelling	doaj-c9d70825284743998dcbe927064d28242021-05-11T07:14:33ZengNature Publishing GroupNature Communications2041-17232017-11-018111210.1038/s41467-017-01250-8Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopyYicong Wu0Abhishek Kumar1Corey Smith2Evan Ardiel3Panagiotis Chandris4Ryan Christensen5Ivan Rey-Suarez6Min Guo7Harshad D. Vishwasrao8Jiji Chen9Jianyong Tang10Arpita Upadhyaya11Patrick J. La Riviere12Hari Shroff13Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthDepartment of Radiology, University of ChicagoSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthAdvanced Imaging and Microscopy Resource, National Institutes of HealthAdvanced Imaging and Microscopy Resource, National Institutes of HealthJT Scientific Consulting LLCBiophysics Program, University of MarylandDepartment of Radiology, University of ChicagoSection on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthLight-sheet fluorescence microscopy enables high resolution imaging of biological samples. Here the authors use reflective coverslips to obtain multiple sample views simultaneously, improving the speed of acquisition and resolution compared to dual-view selective plane illumination microscopy.https://doi.org/10.1038/s41467-017-01250-8
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yicong Wu Abhishek Kumar Corey Smith Evan Ardiel Panagiotis Chandris Ryan Christensen Ivan Rey-Suarez Min Guo Harshad D. Vishwasrao Jiji Chen Jianyong Tang Arpita Upadhyaya Patrick J. La Riviere Hari Shroff
spellingShingle	Yicong Wu Abhishek Kumar Corey Smith Evan Ardiel Panagiotis Chandris Ryan Christensen Ivan Rey-Suarez Min Guo Harshad D. Vishwasrao Jiji Chen Jianyong Tang Arpita Upadhyaya Patrick J. La Riviere Hari Shroff Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy Nature Communications
author_facet	Yicong Wu Abhishek Kumar Corey Smith Evan Ardiel Panagiotis Chandris Ryan Christensen Ivan Rey-Suarez Min Guo Harshad D. Vishwasrao Jiji Chen Jianyong Tang Arpita Upadhyaya Patrick J. La Riviere Hari Shroff
author_sort	Yicong Wu
title	Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
title_short	Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
title_full	Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
title_fullStr	Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
title_full_unstemmed	Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
title_sort	reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2017-11-01
description	Light-sheet fluorescence microscopy enables high resolution imaging of biological samples. Here the authors use reflective coverslips to obtain multiple sample views simultaneously, improving the speed of acquisition and resolution compared to dual-view selective plane illumination microscopy.
url	https://doi.org/10.1038/s41467-017-01250-8
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Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy

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